Site-specific assembly of DNA-based photonic wires by using programmable polyamides.
Site-specific assembly of DNA-based photonic wires by using programmable polyamides.
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DOI：
10.1002/anie.201006735
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发表时间：
2011-03-14
影响因子：
16.6
通讯作者：
Burley, Glenn A.
中科院分区：
文献类型：
--
作者：
Su, Wu;Schuster, Markus;Bagshaw, Clive R.;Rant, Ulrich;Burley, Glenn A.
具有纳米级精度的功能材料的自下而上组织是未来传感器，机器和设备开发的中心目标。[1]这些发展的基础是DNA引导过程的快速发展，[2]它独特地提供了一个可寻址的模板，用于将分子组分放置在离散的一维、二维和三维组装中。[1d然而，将诸如荧光团的多个分子组分可再现地并且以良好控制的分子距离掺入到这样的阵列中仍然是一个艰巨的挑战。[4]以前的研究已经强调了基于DNA的光子线系统的实用性，[5]然而，这些系统遭受与适当的通信组件沿着或在DNA双链体内的低效自组装相关的能量转移（ET）损失。为了克服这些低效率和提供的手段来构建日益复杂和addressability的模块化的光子线，我们需要的工具，使精细的控制的位置和空间排列的荧光团在DNA duplex.Pyrrole-imidazole聚酰胺（PAs）是一类小分子配体，提供这样的控制。PA结合在双链体DNA的小沟内，使得能够以高结合亲和力（纳摩尔至亚纳摩尔）和特异性靶向6至10个碱基对序列。[6]我们推测，将PA的高度特异性识别特性与荧光团中继连接起来，将有可能构建一个可寻址的光子线模型系统，其中第一次可以控制PA的精确嵌入。
The bottom-up organization of functional materials with nanoscale precision is a central goal for the development of future sensors, machines, and devices.[1] Underpinning these developments has been the rapid progression of DNA-guided processes,[2] which uniquely provides an addressable template for the placement of molecular components in discrete one-, two-, and three-dimensional assemblies.[1d, 3] However, the incorporation of multiple molecular components such as fluorophores into such arrays reproducibly and with wellcontrolled molecular distances remains a formidable challenge.[4] Previous studies have highlighted the utility of DNA-based photonic wire systems,[5] however, these systems suffer from energy transfer (ET) losses associated with inefficient self-assembly of the appropriate communicating components along or within the DNA duplexes. In order to overcome these inefficiencies and provide the means to construct modular photonic wires of increasing complexity and addressability, we require tools which enable the exquisite control of the location and spatial arrangement of fluorophores within a DNA duplex.Pyrrole-imidazole polyamides (PAs) are a class of smallmolecule ligands which provide such control. PAs bind within the minor groove of duplex DNA, enabling one to target 6 to 10 base pair sequences with high binding affinity (nanomolar to subnanomolar) and specificity.[6] We surmised that interfacing the highly specific recognition properties of PAs with fluorophore relays, it would be possible to construct an addressable photonic wire model system where for the first time one could control the precise intercalation of a
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