Analysis of banana transcriptome and global gene expression profiles in banana roots in response to infection by race 1 and tropical race 4 of Fusarium oxysporum f. sp. cubense.
Analysis of banana transcriptome and global gene expression profiles in banana roots in response to infection by race 1 and tropical race 4 of Fusarium oxysporum f. sp. cubense.
复制标题
氧化镰刀菌的感染和热带种族4的响应香蕉根中的香蕉转录组和全球基因表达谱分析。 sp。 cubense。
DOI：
10.1186/1471-2164-14-851
复制
发表时间：
2013-12-05
期刊：
影响因子：
4.4
通讯作者：
Peng M
中科院分区：
文献类型：
--
作者：
Li C;Shao J;Wang Y;Li W;Guo D;Yan B;Xia Y;Peng M
卡文迪什，最广泛种植的香蕉品种，是相对抗尖孢镰刀菌1号小种。在世纪上半叶引起大面积传播的巴拿马病（Panama disease）的古巴黑胫病（Foc 1），对威胁世界香蕉生产的古巴黑胫病热带4号小种（FocTR 4）敏感。二倍体香蕉（Musa acuminata）是大多数三倍体香蕉品种的祖先，其基因组最近已被测序。香蕉转录组的可用性将非常有用的改进香蕉基因组注释和生物学研究。了解不同Foc小种感染对宿主基因表达的影响，将有助于理解宿主对感染的反应。将来自卡文迪什栽培品种的不同器官的RNA样品合并用于使用Illumina技术进行深度测序。对香蕉转录组的分析导致鉴定出超过842个未被Musa基因组计划注释的基因。从转录组数据中鉴定出大量的简单核苷酸多态性（SNP）和短插入和缺失（indels）。用表达GFP的Foc 1和FocTR 4监测感染过程。Foc 1和FocTR 4都能在接种后的头两天内侵入香蕉根并扩散到根维管组织中。数字基因表达谱分析表明，Foc 1和FocTR 4的感染在香蕉根中引起了非常相似的基因表达谱变化。Foc感染导致许多已知的防御相关基因的诱导。两个基因编码的乙烯生物合成酶ACC氧化酶和几个乙烯响应转录因子（ERF）的强烈诱导的基因之间的焦点1和焦点TR 4。Foc 1和FocTR 4都能在感染初期进入香蕉根的维管系统，并且它们的感染导致了香蕉根中相似的基因表达谱。转录组分析表明，乙烯合成和信号转导途径被激活，响应于Foc感染。本文的在线版本（doi：10.1186/1471-2164-14-851）包含补充材料，可供授权用户使用。
Cavendish, the most widely grown banana cultivar, is relatively resistant to Race 1 of Fusarium oxysporum f. sp. cubense (Foc1) which caused widespread Panama disease during the first half of the 20th century but is susceptible to Tropical Race 4 of Foc (Foc TR4) which is threatening world banana production. The genome of the diploid species Musa acuminata which is the ancestor of a majority of triploid banana cultivars has recently been sequenced. Availability of banana transcriptomes will be highly useful for improving banana genome annotation and for biological research. The knowledge of global gene expression patterns influenced by infection of different Foc races will help to understand the host responses to the infection. RNA samples from different organs of the Cavendish cultivar were pooled for deep sequencing using the Illumina technology. Analysis of the banana transcriptome led to identification of over 842 genes that were not annotated by the Musa genome project. A large number of simple nucleotide polymorphisms (SNPs) and short insertions and deletion (indels) were identified from the transcriptome data. GFP-expressing Foc1 and Foc TR4 were used to monitor the infection process. Both Foc1 and Foc TR4 were found to be able to invade banana roots and spread to root vascular tissues in the first two days following inoculation. Digital gene expression (DGE) profiling analysis reveal that the infection by Foc1 and Foc TR4 caused very similar changes in the global gene expression profiles in the banana roots during the first two days of infection. The Foc infection led to induction of many well-known defense-related genes. Two genes encoding the ethylene biosynthetic enzyme ACC oxidase and several ethylene-responsive transcription factors (ERF) were among the strongly induced genes by both Foc1 and Foc TR4. Both Foc1 and Foc TR4 are able to spread into the vascular system of banana roots during the early infection process and their infection led to similar gene expression profiles in banana roots. The transcriptome profiling analysis indicates that the ethylene synthetic and signalling pathways were activated in response to the Foc infection. The online version of this article (doi:10.1186/1471-2164-14-851) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
64.8
作者：
Chinchilla, Delphine;Zipfel, Cyril;Boller, Thomas
通讯作者：
Boller, Thomas
影响因子：
64.5
作者：
Li, J;Wen, JQ;Walker, JC
通讯作者：
Walker, JC
影响因子：
4.5
作者：
Hwang, SC;Ko, WH
通讯作者：
Ko, WH
影响因子：
3.3
作者：
Diener, AC;Ausubel, FM
通讯作者：
Ausubel, FM
影响因子：
11.6
作者：
Lai, Zhibing;Li, Ying;Chen, Zhixiang
通讯作者：
Chen, Zhixiang