Electro-focusing liquid extractive surface analysis (EF-LESA) coupled to mass spectrometry.
Electro-focusing liquid extractive surface analysis (EF-LESA) coupled to mass spectrometry.
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DOI：
10.1021/ac4035136
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发表时间：
2014-04-01
影响因子：
7.4
通讯作者：
Godfrey, A. Ruth
中科院分区：
文献类型：
--
作者：
Brenton, A. Gareth;Godfrey, A. Ruth
通过与质谱仪连接的液体采样装置分析表面的化学组成是有吸引力的，因为可以以良好的灵敏度和选择性连续监测样品流。已经构造了取样探针，其获取表面的离散液体样品（通常<100 nL）。它结合了静电透镜系统，包括三个电极，静电和脉冲电压施加到该电极以形成锥形“液体尖端”，用于溶解表面处的分析物。原型系统演示了0.093 mm2的空间分辨率。控制液体尖端和表面之间的接触时间以使浸提标准化。在不锈钢表面上不同的分析物浓度的校准图已被测量，连同探针的再现性，携带，和回收。亮氨酸脑啡肽包被的表面表现出良好的线性（R2 = 0.9936），与90%的回收率和38 fmol的检测限每个单点采样。该探头结构紧凑，可以安装到自动样品分析设备中，具有以良好的空间分辨率快速分析表面的潜力。
Analysis of the chemical composition of surfaces by liquid sampling devices interfaced to mass spectrometry is attractive as the sample stream can be continuously monitored at good sensitivity and selectivity. A sampling probe has been constructed that takes discrete liquid samples (typically <100 nL) of a surface. It incorporates an electrostatic lens system, comprising three electrodes, to which static and pulsed voltages are applied to form a conical “liquid tip”, employed to dissolve analytes at a surface. A prototype system demonstrates spatial resolution of 0.093 mm2. Time of contact between the liquid tip and the surface is controlled to standardize extraction. Calibration graphs of different analyte concentrations on a stainless surface have been measured, together with the probe’s reproducibility, carryover, and recovery. A leucine enkephalin-coated surface demonstrated good linearity (R2 = 0.9936), with a recovery of 90% and a limit of detection of 38 fmol per single spot sampled. The probe is compact and can be fitted into automated sample analysis equipment having potential for rapid analysis of surfaces at a good spatial resolution.
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影响因子：
2.3
作者：
Parson, Whitney B.;Koeniger, Stormy L.;Van Berkel, Gary J.
通讯作者：
Van Berkel, Gary J.
影响因子：
7.4
作者：
Laskin, Julia;Heath, Brandi S.;Roach, Patrick J.;Cazares, Lisa;Semmes, O. John
通讯作者：
Semmes, O. John
影响因子：
2
作者：
Walworth, Matthew J.;ElNaggar, Mariam S.;Van Berkel, Gary J.
通讯作者：
Van Berkel, Gary J.
影响因子：
1.8
作者：
WILM, MS;MANN, M
通讯作者：
MANN, M
影响因子：
1.8
作者：
Yogi, Osamu;Kawakami, Tomonori;Mizuno, Akira
通讯作者：
Mizuno, Akira