Genetic diversity and population structure of Miscanthus sinensis germplasm in China.
Genetic diversity and population structure of Miscanthus sinensis germplasm in China.
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DOI：
10.1371/journal.pone.0075672
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Peng J
中科院分区：
文献类型：
--
作者：
Zhao H;Wang B;He J;Yang J;Pan L;Sun D;Peng J
芒草是一种多年生根茎状C4草，原产于东亚。芒草具有生物质产量大、木质纤维素成分高、对辐射、养分和水分利用效率高、耐胁迫等特点，是一种极具潜力的优质生物能源作物。尽管同源三倍体麦草×giganteus具有很高的生物量生产潜力，但需要探索其他选择，以改善麦草×giganteus狭窄的遗传基础，并开发其他芒草物种，包括麦草（2n = 2x = 38），作为生物能源作物。本研究从中国广泛的地理分布地区收集了大量的459份中华短腿草材料，利用23个SSR标记对其进行了基因分型。遗传多样性和群体结构进行了评估。该种质具有较高的遗传多样性和分化性，共有115个等位基因，多态性率为0.77,Nei’s遗传多样性指数（He）为0.32，多态性信息含量（PIC）为0.26。种质资源的聚类与自然地理分布基本一致。AMOVA分析和遗传距离分析证实了中华水杨种质资源的遗传分化，并将其划分为5个聚类或亚群。亚群间遗传变异显著，表明亚群内遗传分化明显，但亚群内遗传变异（83%）远大于亚群间遗传变异（17%）。结果表明，在300份中华鲟材料中，多个性状存在显著的表型变异。发现9个SSR标记与抽穗日期和生物量产量相关。结果表明，丰富的中国芒草种质资源和新鉴定的SSR标记对选育具有理想生物能源性状的芒草品种具有重要价值。
Miscanthus is a perennial rhizomatous C4 grass native to East Asia. Endowed with great biomass yield, high ligno-cellulose composition, efficient use of radiation, nutrient and water, as well as tolerance to stress, Miscanthus has great potential as an excellent bioenergy crop. Despite of the high potential for biomass production of the allotriploid hybrid M. ×giganteus, derived from M. sacchariflorus and M. sinensis, other options need to be explored to improve the narrow genetic base of M. ×giganteus, and also to exploit other Miscanthus species, including M. sinensis (2n = 2x = 38), as bioenergy crops. In the present study, a large number of 459 M. sinensis accessions, collected from the wide geographical distribution regions in China, were genotyped using 23 SSR markers transferable from Brachypodium distachyon. Genetic diversity and population structure were assessed. High genetic diversity and differentiation of the germplasm were observed, with 115 alleles in total, a polymorphic rate of 0.77, Nei’s genetic diversity index (He) of 0.32 and polymorphism information content (PIC) of 0.26. Clustering of germplasm accessions was primarily in agreement with the natural geographic distribution. AMOVA and genetic distance analyses confirmed the genetic differentiation in the M. sinensis germplasm and it was grouped into five clusters or subpopulations. Significant genetic variation among subpopulations indicated obvious genetic differentiation in the collections, but within-subpopulation variation (83%) was substantially greater than the between-subpopulation variation (17%). Considerable phenotypic variation was observed for multiple traits among 300 M. sinensis accessions. Nine SSR markers were found to be associated with heading date and biomass yield. The diverse Chinese M. sinensis germplasm and newly identified SSR markers were proved to be valuable for breeding Miscanthus varieties with desired bioenergy traits.
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影响因子：
6
作者：
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通讯作者：
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影响因子：
1.9
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1111/j.1471-8286.2006.01380.x
发表时间：
2006-09-01
期刊：
MOLECULAR ECOLOGY NOTES
影响因子：
--
作者：
Ehrich, Dorothee
通讯作者：
Ehrich, Dorothee