Analysis of aneuploid lines of bread wheat to map chromosomal locations of genes controlling root hair length
Analysis of aneuploid lines of bread wheat to map chromosomal locations of genes controlling root hair length
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分析面包小麦的非整倍体系以绘制控制根毛长度的基因的染色体位置图
DOI：
10.1093/aob/mcx030
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发表时间：
2017-06
期刊：
影响因子：
4.2
通讯作者：
Delhaize E
中科院分区：
文献类型：
--
作者：
Rathjen T.;Forrest K.;Hayden M.;Delhaize E
背景和目的长的根毛能够有效地吸收移动的较差的营养物质，如磷。绘制控制根毛长度的基因的染色体位置可以帮助利用作物中的自然变异来开发改良的品种。利用小麦品种“中国春”的遗传资源定位控制根毛长度的基因。方法以根鞘大小为指标，采用快速筛选法筛选“中国春”非整倍体材料，并对筛选出的材料进行根毛长度的测定，以鉴定含有根毛长度控制基因的染色体。然后筛选出一系列候选染色体不同比例缺失的品系，以更准确地绘制根毛基因座。用90 000个单核苷酸多态性（SNP）阵列分析了5A染色体缺失的品系。在低磷和高磷条件下，比较了一个缺失系与整倍体‘中国春’的磷吸收效率。染色体1A、1D和5A被发现含有控制根毛长度的基因。90 000 SNP阵列鉴定了位于染色体5A上的两个控制根毛长度的候选基因。在染色体5A中具有缺失的品系具有约为0.000000的根毛。20%，但这不足以降低其PAE。结论根鞘大小的快速筛选使控制根毛长度的染色体区域被定位在小麦品种'中国春'中，随后的SNP阵列分析确定了控制根毛长度的候选基因。整倍体“中国春”和快速筛选中鉴定的缺失系之间的根毛长度差异仍然明显，尽管减弱，当幼苗生长在完全施肥的土壤上时。
Background and Aims Long root hairs enable the efficient uptake of poorly mobile nutrients such as phosphorus. Mapping the chromosomal locations of genes that control root hair length can help exploit the natural variation within crops to develop improved cultivars. Genetic stocks of the wheat cultivar ‘Chinese Spring’ were used to map genes that control root hair length. Methods Aneuploid stocks of ‘Chinese Spring’ were screened using a rapid method based on rhizosheath size and then selected lines were assayed for root hair length to identify chromosomes harbouring genes controlling root hair length. A series of lines with various fractional deletions of candidate chromosomes were then screened to map the root hair loci more accurately. A line with a deletion in chromosome 5A was analysed with a 90 000 single nucleotide polymorphism (SNP) array. The phosphorus acquisition efficiency (PAE) of one deletion line was compared with that of euploid ‘Chinese Spring’ by growing the seedlings in pots at low and luxury phosphorus supplies. Key Results Chromosomes 1A, 1D and 5A were found to harbour genes controlling root hair length. The 90 000 SNP array identified two candidate genes controlling root hair length located on chromosome 5A. The line with a deletion in chromosome 5A had root hairs that were approx. 20 % shorter than euploid ‘Chinese Spring’, but this was insufficient to reduce its PAE. Conclusions A rapid screen for rhizosheath size enabled chromosomal regions controlling root hair length to be mapped in the wheat cultivar ‘Chinese Spring’ and subsequent analysis with an SNP array identified candidate genes controlling root hair length. The difference in root hair length between euploid ‘Chinese Spring’ and a deletion line identified in the rapid screen was still apparent, albeit attenuated, when the seedlings were grown on a fully fertilized soil.
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影响因子：
6.9
作者：
Tanaka N;Kato M;Tomioka R;Kurata R;Fukao Y;Aoyama T;Maeshima M
通讯作者：
Maeshima M
影响因子：
3.1
作者：
E. Whelan;G. B. Schaalje
通讯作者：
E. Whelan;G. B. Schaalje
影响因子：
7.2
作者：
Nestler, Josefine;Liu, Sanzhen;Hochholdinger, Frank
通讯作者：
Hochholdinger, Frank
影响因子：
3
作者：
Bates, TR;Lynch, JP
通讯作者：
Lynch, JP
影响因子：
5.4
作者：
Y. Henry;J. Marcotte;J. Buyser
通讯作者：
Y. Henry;J. Marcotte;J. Buyser