Characterization of corticotropin-releasing hormone binding protein in human plasma by chemical cross-linking and its binding during pregnancy.
Characterization of corticotropin-releasing hormone binding protein in human plasma by chemical cross-linking and its binding during pregnancy.
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通过化学交联表征人血浆中促肾上腺皮质激素释放激素结合蛋白及其在妊娠期间的结合。
DOI：
--
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发表时间：
1988
影响因子：
5.8
通讯作者：
Kazuo Shizume
中科院分区：
文献类型：
--
作者：
Toshihiro Suda;Mitsutoshi Iwashita;F. Tozawa;T. Ushiyama;N. Tomori;T. Sumitomo;Y. Nakagami;Hiroshi Demura;Kazuo Shizume
A human plasma CRH-binding protein (CRH-BP) was identified and characterized by chemical cross-linking of 125I-Tyr-hCRH to human plasma using disuccinimidyl suberate. The apparent mol wt of the cross-linked complex determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by autoradiography was approximately 43,000. The mol wt was slightly lower in the nonreduced state, suggesting the presence of intramolecular disulfide bonds. Subtracting the mol wt of 125I-Tyr-CRH, the BP appeared to have a mol wt of approximately 38,000. Binding was specific since the appearance of the 43,000 dalton band was not affected by unlabeled ACTH, vasopressin, serum albumin, or gamma-globulin, but was inhibited by unlabeled hCRH dose dependently. Pretreatment of plasma with 0.1 mol/L HCl, 0.01 mol/L NaOH, 10 mmol/L dithiothreitol, or trypsin before cross-linking abolished its ability to bind 125I-Tyr-hCRH. Rat, rabbit, or goat plasma or human cerebrospinal fluid did not bind 125I-Tyr-CRH. It is unlikely that CRH-BP is a CRH receptor, because the estimated mol wt of the CRH-BP is smaller than the reported size of CRH receptors, and the CRH-BP did not bind to ovine CRH. The binding of 125I-Tyr-CRH to CRH-BP decreased in the third trimester of pregnancy, when plasma CRH levels were markedly elevated. However, after dissociating endogenous CRH from the CRH-BP, the binding was almost the same as in nonpregnant subjects. In addition, CRH-BP inhibited CRH-induced ACTH secretion from cultured rat anterior pituitary cells. We conclude that most of the increased plasma CRH found in pregnant women is bound to CRH-BP, and so is inactive, therefore plasma ACTH levels do not increase to above the normal range.
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AtT-20小鼠垂体肿瘤细胞质膜中促肾上腺皮质激素释放因子结合蛋白的鉴定及其地塞米松的调节。
影响因子：
4.8
作者：
Rosendale,BE;Jarrett,DB;Robinson,AG
通讯作者：
Robinson,AG
影响因子：
4.8
作者：
M. Perrin;Yaira Haas;J. Rivier;W. Vale
通讯作者：
M. Perrin;Yaira Haas;J. Rivier;W. Vale
通过化学交联鉴定和表征垂体促肾上腺皮质激素释放因子结合蛋白。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nishimura,E;Billestrup,N;Perrin,M;Vale,W
通讯作者：
Vale,W
影响因子：
5.8
作者：
GOLAND, RS;WARDLAW, SL;FRANTZ, AG
通讯作者：
FRANTZ, AG
正常人血浆中人促肾上腺皮质激素释放激素的特异性高亲和力结合蛋白。
DOI：
10.1016/0006-291x(87)91369-6
发表时间：
1987
影响因子：
3.1
作者：
Orth,DN;Mount,CD
通讯作者：
Mount,CD