Mechanisms involved in extraterritorial facial pain following cervical spinal nerve injury in rats.
Mechanisms involved in extraterritorial facial pain following cervical spinal nerve injury in rats.
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DOI：
10.1186/1744-8069-7-12
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发表时间：
2011-02-10
期刊：
影响因子：
3.3
通讯作者：
Iwata K
中科院分区：
文献类型：
--
作者：
Kobayashi A;Shinoda M;Sessle BJ;Honda K;Imamura Y;Hitomi S;Tsuboi Y;Okada-Ogawa A;Iwata K
本研究的目的是阐明颈髓神经损伤后口面部疼痛异常的神经机制。本实验观察了高位颈神经切断(CNX)大鼠三叉神经脊束核尾侧亚核(Vc)和上颈段脊髓背角(C1-C2)神经元的突触行为、磷酸化细胞外信号调节激酶(PERK)的表达和星形胶质细胞的激活。与Sham大鼠相比，CNX大鼠对侧面皮肤机械刺激的头部撤退阈值和对面部侧面皮肤加热的头部撤退潜伏期分别显着降低和缩短。这些效应在CNX后1d内明显，并持续到21d以上。伤害性和非伤害性机械或热刺激面部外侧皮肤后第7天，CNX大鼠Vc和C1-C2浅层的Perk样免疫反应(LI)细胞数明显多于Sham大鼠。机械刺激和热刺激后，Vc和C1-C2的Perk-LI细胞出现两个峰。C_1-C_2区的PERK-LI细胞数量呈强度依赖性，并随面部机械刺激和热刺激的增加而增加。鞘内注射可逆转热刺激引起的头部退缩潜伏期和机械刺激引起的头部退缩阈值的降低。给予MAPK/ERK激酶1/2抑制剂PD98059。CNX大鼠激活的星形胶质细胞面积明显增加(CNX后第7天)。伤害性和非伤害性机械刺激后，大鼠面部的热刺激和机械刺激行为明显受到抑制，Vc和C1-C2的Perk-LI细胞数量也明显减少。给予星形胶质细胞抑制剂氟乙酸酯。本研究结果表明，颈神经损伤后面部外侧皮肤发生机械性痛敏和热痛敏，并提示Vc和C1-C2神经元ERK的磷酸化和星形胶质细胞的激活参与了颈神经损伤后口腔面外痛的发生。
The aim of this study is to clarify the neural mechanisms underlying orofacial pain abnormalities after cervical spinal nerve injury. Nocifensive behavior, phosphorylated extracellular signal-regulated kinase (pERK) expression and astroglial cell activation in the trigeminal spinal subnucleus caudalis (Vc) and upper cervical spinal dorsal horn (C1-C2) neurons were analyzed in rats with upper cervical spinal nerve transection (CNX). The head withdrawal threshold to mechanical stimulation of the lateral facial skin and head withdrawal latency to heating of the lateral facial skin were significantly lower and shorter respectively in CNX rats compared to Sham rats. These nocifensive effects were apparent within 1 day after CNX and lasted for more than 21 days. The numbers of pERK-like immunoreactive (LI) cells in superficial laminae of Vc and C1-C2 were significantly larger in CNX rats compared to Sham rats following noxious and non-noxious mechanical or thermal stimulation of the lateral facial skin at day 7 after CNX. Two peaks of pERK-LI cells were observed in Vc and C1-C2 following mechanical and heat stimulation of the lateral face. The number of pERK-LI cells in C1-C2 was intensity-dependent and increased when the mechanical and heat stimulations of the face were increased. The decrements of head withdrawal latency to heat and head withdrawal threshold to mechanical stimulation were reversed during intrathecal (i.t.) administration of MAPK/ERK kinase 1/2 inhibitor PD98059. The area of activated astroglial cells was significantly higher in CNX rats (at day 7 after CNX). The heat and mechanical nocifensive behaviors were significantly depressed and the number of pERK-LI cells in Vc and C1-C2 following noxious and non-noxious mechanical stimulation of the face was also significantly decreased following i.t. administration of the astroglial inhibitor fluoroacetate. The present findings have demonstrated that mechanical allodynia and thermal hyperalgesia occur in the lateral facial skin after CNX and also suggest that ERK phosphorylation of Vc and C1-C2 neurons and astroglial cell activation are involved in orofacial extraterritorial pain following cervical nerve injury.
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DOI：
10.1523/jneurosci.3365-09.2009
发表时间：
2009-09-09
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Okada-Ogawa A;Suzuki I;Sessle BJ;Chiang CY;Salter MW;Dostrovsky JO;Tsuboi Y;Kondo M;Kitagawa J;Kobayashi A;Noma N;Imamura Y;Iwata K
通讯作者：
Iwata K
影响因子：
3.5
作者：
Pezet, S;Malcangio, M;McMahon, SB
通讯作者：
McMahon, SB
影响因子：
7.4
作者：
Piao, ZG;Cho, IH;Oh, SB
通讯作者：
Oh, SB
影响因子：
2.5
作者：
PRICE, DD;HAYASHI, H;RUDA, MA
通讯作者：
RUDA, MA
影响因子：
2.5
作者：
Saito, Kimiko;Hitomi, Suzuro;Iwata, Koichi
通讯作者：
Iwata, Koichi