Correlation of T2R38 taste phenotype and in vitro biofilm formation from nonpolypoid chronic rhinosinusitis patients.
Correlation of T2R38 taste phenotype and in vitro biofilm formation from nonpolypoid chronic rhinosinusitis patients.
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DOI：
10.1002/alr.21803
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发表时间：
2016-08
影响因子：
6.4
通讯作者：
Cohen NA
中科院分区：
文献类型：
--
作者：
Adappa ND;Truesdale CM;Workman AD;Doghramji L;Mansfield C;Kennedy DW;Palmer JN;Cowart BJ;Cohen NA
在慢性鼻窦炎（CRS）患者的鼻窦标本中发现了生物膜。越来越多的证据表明，生物膜有助于治疗上抑制CRS。最近，苦味受体T2R38已被牵连在调节鼻腔鼻窦粘膜先天性免疫反应。TAS2R38基因多态性影响受体功能，并有助于在鼻窦先天防御中观察到的变化，以及反映在对苦味化合物苯硫脲（PTC）的味觉敏感性中的味觉感知。在患有活动性感染或炎症的CRS患者群体中，我们试图确定T2 R38表型与体外生物膜形成之间是否存在相关性。从具有持续性炎症或粘膜糜烂证据的CRS（±息肉）患者中前瞻性获得内窥镜引导的鼻窦拭子。用改良的卡尔加里生物膜检测试验评估体外生物膜形成。患者的苦味受体T2R38的表型（功能性）表达用包括化合物PTC的味觉试验来评价。使用线性回归来确定CRS患者的平均体外生物膜形成水平和平均PTC味觉测试强度评级之间的显著性水平。从59名患者中获得鼻窦拭子，其中59个样品中的42个证明了体外生物膜形成。分析显示，所有患者的体外生物膜形成和PTC味觉强度等级之间存在负线性相关（p = 0.019）。这种关联仅由非息肉样CRS患者驱动（p = 0.0026）。在非息肉样CRS患者中，鼻腔鼻窦临床分离株的体外生物膜形成与PTC味觉敏感性呈负相关。
Sinonasal biofilms have been demonstrated in specimens collected from chronic rhinosinusitis (CRS) patients. Mounting evidence suggests that biofilms contribute to therapeutically recalcitrant CRS. Recently, the bitter taste receptor T2R38 has been implicated in the regulation of the sinonasal mucosal innate immune response. TAS2R38 gene polymorphisms affect receptor functionality and contribute to variations seen in sinonasal innate defense as well as taste perception reflected in gustatory sensitivity to the bitter compound phenylthiocarbamide (PTC). In a population of CRS patients with active infection or inflammation, we sought to determine if a correlation between T2R38 phenotype and in vitro biofilm formation existed. Endoscopically guided sinonasal swabs were obtained prospectively from CRS (±polyp) patients with evidence of persistent inflammation or mucopurulence. In vitro biofilm formation was assessed with a modified Calgary Biofilm Detection Assay. Patients' phenotypic (functional) expression of the bitter taste receptor T2R38 was evaluated with a taste test including the compound PTC. Linear regression was used to determine the level of significance between mean in vitro biofilm formation levels and mean PTC taste test intensity ratings across CRS patients. Sinonasal swabs were obtained from 59 patients, with 42 of the 59 samples demonstrating in vitro biofilm formation. Analysis revealed an inverse linear association between in vitro biofilm formation and PTC taste intensity ratings (p = 0.019) for all patients. This association was exclusively driven by nonpolypoid CRS patients (p = 0.0026). In vitro biofilm formation from sinonasal clinical isolates is inversely correlated with PTC taste sensitivity in nonpolypoid CRS patients.
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影响因子：
6.4
作者：
Adappa ND;Farquhar D;Palmer JN;Kennedy DW;Doghramji L;Morris SA;Owens D;Mansfield C;Lysenko A;Lee RJ;Cowart BJ;Reed DR;Cohen NA
通讯作者：
Cohen NA
影响因子：
3.7
作者：
Hawn TR;Scholes D;Li SS;Wang H;Yang Y;Roberts PL;Stapleton AE;Janer M;Aderem A;Stamm WE;Zhao LP;Hooton TM
通讯作者：
Hooton TM
影响因子：
3.4
作者：
Healy, David Y.;Leid, Jeff G.;Hunsaker, Darrell H.
通讯作者：
Hunsaker, Darrell H.
影响因子：
2.6
作者：
Cohen, Noam A.;Zhang, Shaoyan;Woodworth, Bradford A.
通讯作者：
Woodworth, Bradford A.
影响因子：
9.2
作者：
Bufe, B;Breslin, PAS;Meyerhof, W
通讯作者：
Meyerhof, W