Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments.
Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments.
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矮牵牛花衰老过程中ERF转录因子基因的鉴定和表达分析以及对激素处理的反应
DOI：
10.1093/jxb/erq324
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发表时间：
2011-01
影响因子：
6.9
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Liu J;Li J;Wang H;Fu Z;Liu J;Yu Y
乙烯响应元件结合因子（乙烯-responsive element-binding factor， ERF）是植物中最大的转录因子基因家族之一。在拟南芥和水稻中，迄今为止只有少数ERF基因被鉴定。花的衰老与许多花的乙烯产量增加有关。然而，ERF基因在花衰老中的作用尚未见报道。本研究从矮牵牛花中克隆了13个ERF cdna。根据序列特征，这些pherf可分为已知的12个ERF家族中的4个。他们预测的氨基酸序列与其他植物物种的erf相似。在矮牵牛花冠和雌蕊中进行了PhERF mrna的表达分析。13个PhERF基因在花自然衰老过程中表现出不同的表达模式和表达水平。外源乙烯加速了各种PhERF基因的转录，硫代硫酸银（STS）降低了花冠和雌蕊中几种PhERF基因的转录。第VII组的PhERF基因与花瓣和雌蕊乙烯产量的增加密切相关，并可能与矮牵牛花的衰老密切相关。研究了糖、茉莉酸甲酯和植物激素脱落酸、水杨酸和6-苄基腺嘌呤对不同PhERF转录本的调控作用。利用荧光显微镜对两个PhERF蛋白（PhERF2和PhERF3）进行功能性核定位信号分析。这些结果支持了矮牵牛花PhERF基因在矮牵牛花衰老过程中的转录调控作用。
Ethylene-responsive element-binding factor (ERF) genes constitute one of the largest transcription factor gene families in plants. In Arabidopsis and rice, only a few ERF genes have been characterized so far. Flower senescence is associated with increased ethylene production in many flowers. However, the characterization of ERF genes in flower senescence has not been reported. In this study, 13 ERF cDNAs were cloned from petunia. Based on the sequence characterization, these PhERFs could be classified into four of the 12 known ERF families. Their predicted amino acid sequences exhibited similarities to ERFs from other plant species. Expression analyses of PhERF mRNAs were performed in corollas and gynoecia of petunia flower. The 13 PhERF genes displayed differential expression patterns and levels during natural flower senescence. Exogenous ethylene accelerates the transcription of the various PhERF genes, and silver thiosulphate (STS) decreased the transcription of several PhERF genes in corollas and gynoecia. PhERF genes of group VII showed a strong association with the rise in ethylene production in both petals and gynoecia, and might be associated particularly with flower senescence in petunia. The effect of sugar, methyl jasmonate, and the plant hormones abscisic acid, salicylic acid, and 6-benzyladenine in regulating the different PhERF transcripts was investigated. Functional nuclear localization signal analyses of two PhERF proteins (PhERF2 and PhERF3) were carried out using fluorescence microscopy. These results supported a role for petunia PhERF genes in transcriptional regulation of petunia flower senescence processes.
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影响因子：
6.9
作者：
El-Sharkawy I;Sherif S;Mila I;Bouzayen M;Jayasankar S
通讯作者：
Jayasankar S
影响因子：
5.1
作者：
JONES, ML;LARSEN, PB;WOODSON, WR
通讯作者：
WOODSON, WR
影响因子：
4.2
作者：
Müller, R;Stummann, BM;Serek, M
通讯作者：
Serek, M
影响因子：
6.4
作者：
Hunter, DA;Ferrante, A;Reid, MS
通讯作者：
Reid, MS
影响因子：
6.9
作者：
Hoeberichts, Frank A.;van Doorn, Wouter G.;van Wordragen, Monique F.
通讯作者：
van Wordragen, Monique F.