The 5' untranslated region of the soybean cytosolic glutamine synthetase β(1) gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants.
The 5' untranslated region of the soybean cytosolic glutamine synthetase β(1) gene contains prokaryotic translation initiation signals and acts as a translational enhancer in plants.
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DOI：
10.1007/s00438-012-0724-6
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Sengupta-Gopalan C
中科院分区：
文献类型：
--
作者：
Ortega JL;Wilson OL;Sengupta-Gopalan C
谷氨酰胺合成酶（GS）催化谷氨酸和氨合成谷氨酰胺。在植物中，它以两种主要亚型存在，即胞质形式 (GS1) 和核编码叶绿体形式。本文的重点是确定 GS1 基因 5'UTR 的作用。将带有或不带有 5' 和 3' UTR 的 GS1 基因构建体由组成型启动子驱动，将其渗入烟草叶中，并分析组织的转基因转录物和蛋白质积累。该构建体还在体外转录/翻译系统和大肠杆菌中进行了测试。我们的结果表明，虽然 3' UTR 在转录本的不稳定中发挥作用，但 5' UTR 在植物细胞中充当翻译增强子，但在体外翻译系统中则不然。当将 GS1 基因的 5'UTR 置于报告基因 (uidA) 前面时，与没有 5'UTR 的相同基因构建体相比，农杆菌渗透叶片中的 GUS 表达水平增加了 20 倍。 5'UTR 介导的翻译增强可能是植物中 GS 调节的另一个步骤。 GS1 编码区前面存在 GS1 5' UTR，使其能够在大肠杆菌中进行翻译，这表明该基因在植物和细菌之间的翻译起始机制具有共性。
Glutamine synthetase (GS) catalyzes the synthesis of glutamine from glutamate and ammonia. In plants, it occurs as two major isoforms, a cytosolic form (GS1) and a nuclear encoded chloroplastic form. The focus of this paper is to determine the role of the 5′UTR of a GS1 gene. GS1 gene constructs with and without its 5′ and 3′ UTRs, driven by a constitutive promoter, were agroinfiltrated into tobacco leaves and the tissues were analyzed for both transgene transcript and protein accumulation. The constructs were also tested in an in vitro transcription/translation system and in Escherichia coli. Our results showed that while the 3′ UTR functioned in the destabilization of the transcript, the 5′ UTR acted as a translation enhancer in plant cells but not in the in vitro translation system. The 5′UTR of the GS1 gene when placed in front of a reporter gene (uidA), showed a 20-fold increase in the level of GUS expression in agroinfiltrated leaves when compared to the same gene construct without the 5′UTR. The 5′UTR-mediated translational enhancement is probably another step in the regulation of GS in plants. The presence of the GS1 5′ UTR in front of the GS1 coding region allowed for its translation in E. coli suggesting the commonality of the translation initiation mechanism for this gene between plants and bacteria.
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影响因子：
4.9
作者：
Jimenez-Lopez，Sara;Mancera-Martinez，Eder;Sanchez de Jimenez，Estela
通讯作者：
Sanchez de Jimenez，Estela
影响因子：
56.9
作者：
DASSARMA, S;TISCHER, E;GOODMAN, HM
通讯作者：
GOODMAN, HM
影响因子：
14.9
作者：
Kawaguchi R;Bailey-Serres J
通讯作者：
Bailey-Serres J
影响因子：
5.1
作者：
Carvalho, H;Sunkel, C;Cullimore, JV
通讯作者：
Cullimore, JV
影响因子：
7.2
作者：
Finnemann, J;Schjoerring, JK
通讯作者：
Schjoerring, JK