Analgesic dorsal root ganglion field stimulation blocks both afferent and efferent spontaneous activity in sensory neurons of rats with monosodium iodoacetate-induced osteoarthritis.
Analgesic dorsal root ganglion field stimulation blocks both afferent and efferent spontaneous activity in sensory neurons of rats with monosodium iodoacetate-induced osteoarthritis.
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DOI：
10.1016/j.joca.2022.08.008
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发表时间：
2022-11
影响因子：
7
通讯作者：
Pan, B.
中科院分区：
文献类型：
--
作者：
Chao, D.;Tran, H.;Hogan, Q. H.;Pan, B.
慢性关节疼痛在骨关节炎（OA）患者中很常见。非甾体抗炎药和阿片类药物用于缓解OA疼痛，但它们通常效果不佳。背根神经节野刺激（GFS）是一种临床使用的神经调节方法，尽管它不常用于OA疼痛患者。我们在前期使用碘乙酸钠（MIA）诱导的OA大鼠疼痛模型的研究中显示了GFS的镇痛效果。本研究旨在探讨GFS在该模型中的镇痛作用机制。关节内注射MIA诱导骨关节炎后，进行疼痛行为测试。GFS对自发性活动（SA）的影响通过戏弄纤维隐神经、腓肠神经和背根的体内单单位记录进行测试。关节内注射MIA两周后，大鼠出现疼痛样行为。MIA-OA大鼠隐神经束和第三腰椎（L3）背根的体内单单位记录显示，SA的发生率高于注射盐水的对照组大鼠。L3水平的GFS阻断了L3背根SA。MIA-OA降低了从L4背根拨弄的神经束诱导AP发射的点状机械力阈值，而GFS则恢复正常。MIA-OA后，逆行SA（背根反射）增加，可被GFS阻断。这些结果表明，GFS在MIA-OA大鼠中产生镇痛作用，至少部分是通过阻断传入输入，也可能是通过阻断背角的传出活动。
Chronic joint pain is common in patients with osteoarthritis (OA). Non-steroidal anti-inflammatory drugs and opioids are used to relieve OA pain, but they are often inadequately effective. Dorsal root ganglion field stimulation (GFS) is a clinically used neuromodulation approach, although it is not commonly employed for patients with OA pain. GFS showed analgesic effectiveness in our previous study using the monosodium iodoacetate (MIA) - induced OA rat pain model. This study was to evaluate the mechanism of GFS analgesia in this model. After osteoarthritis was induced by intra-articular injection of MIA, pain behavioral tests were performed. Effects of GFS on the spontaneous activity (SA) were tested with in vivo single-unit recordings from teased fiber saphenous nerve, sural nerve, and dorsal root. Two weeks after intra-articular MIA injection, rats developed pain-like behaviors. In vivo single unit recordings from bundles teased from the saphenous nerve and third lumbar (L3) dorsal root of MIA-OA rats showed a higher incidence of SA than those from saline-injected control rats. GFS at the L3 level blocked L3 dorsal root SA. MIA-OA reduced the punctate mechanical force threshold for inducing AP firing in bundles teased from the L4 dorsal root, which reversed to normal with GFS. After MIA-OA, there was increased retrograde SA (dorsal root reflex), which can be blocked by GFS. These results indicate that GFS produces analgesia in MIA-OA rats at least in part by producing blockade of afferent inputs, possibly also by blocking efferent activity from the dorsal horn.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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