Berberine and itraconazole are not synergistic in vitro against Aspergillus fumigatus isolated from clinical patients.
Berberine and itraconazole are not synergistic in vitro against Aspergillus fumigatus isolated from clinical patients.
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小檗碱和伊曲康唑在体外对临床患者分离的烟曲霉没有协同作用
DOI：
10.3390/molecules16119218
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发表时间：
2011-11-03
期刊：
影响因子：
--
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Lei G;Dan H;Jinhua L;Wei Y;Song G;Li W
由于广泛的免疫抑制，烟曲霉菌感染的发生率已变得更加频繁。目前，临床上可用的抗真菌药物数量有限，且大多数药物如伊曲康唑(ICZ)具有毒性和耐药性。黄连素(berberine，BER)是一种临床上主要用于治疗消化系统感染的植物生物碱。我们用BER和ICZ检测了临床分离的烟曲霉菌的体外耐药性。其最小抑菌浓度范围分别为4~256g/m l和0.031~0.250μg/m L。对烟曲霉菌IFM 40808菌株的MIC50值分别为8和0.125μg/mL。利用该菌株对有无BER的巨大菌落进行比较，得出BER能抑制烟曲霉菌丝生长和分生孢子色素产生的结论。两种药物的组合也通过棋盘试验进行测试，以确定它们之间的任何功能相互作用。42个菌株中有32个菌株的FICI值为>；4.0，表明两种药物具有相互拮抗作用。总之，不建议在临床上同时使用BER和ICZ。我们的结果表明，BER可能通过类似ICZ的麦角甾醇生物合成途径来抑制烟曲霉菌。
The incidence of Aspergillus fumigatus infections has become more frequent as a consequence of widespread immunosuppression. At present, the number of available antifungal agents in the clinic is limited, and most of them, such as itraconazole (ICZ), are toxic and show resistance. Berberine (BER) is a plant alkaloid used in the clinic mainly for alimentary infections. We have used BER and ICZ to measure in vitro resistance in A. fumigatus isolated from clinical patients. The minimum inhibitory concentration ranges of BER and ICZ were 4–256 and 0.031–0.250 μg/mL, respectively. In addition, against A. fumigatus IFM 40808 strain, the MIC50 values of BER and ICZ were 8 and 0.125 μg/mL. Using this strain, we compared the giant colonies with or without BER, and concluded that BER could restrain A. fumigatus mycelial growth and conidial pigment production. Combinations of the two drugs were also tested by the checkerboard assay to identify any functional interactions between them. Thirty-two out of 42 isolates had FICI values > 4.0, indicating that two drugs were mutually antagonistic. In conclusion, it is not advised that BER and ICZ be used in the clinic at the same time. Our results indicated that BER may inhibit A. fumigatus through the ergosterol biosynthesis pathway, like ICZ.
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影响因子：
5.2
作者：
Park, KS;Kang, KC;Paik, YK
通讯作者：
Paik, YK
影响因子：
5
作者：
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通讯作者：
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影响因子：
2
作者：
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通讯作者：
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影响因子：
3.6
作者：
Bien CM;Chang YC;Nes WD;Kwon-Chung KJ;Espenshade PJ
通讯作者：
Espenshade PJ
影响因子：
3.4
作者：
Freile, ML;Giannini, F;Enriz, RD
通讯作者：
Enriz, RD