The ultrastructure of spinal cord perivascular spaces: Implications for the circulation of cerebrospinal fluid.
The ultrastructure of spinal cord perivascular spaces: Implications for the circulation of cerebrospinal fluid.
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DOI：
10.1038/s41598-017-13455-4
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发表时间：
2017-10-10
影响因子：
4.6
通讯作者：
Stoodley MA
中科院分区：
文献类型：
--
作者：
Lam MA;Hemley SJ;Najafi E;Vella NGF;Bilston LE;Stoodley MA
血管周围间隙在脑脊液和间质液之间的交换以及CNS中废物的清除中起着关键作用，但其精确的解剖学组成尚未得到很好的描述。本研究的目的是用透射电镜观察健康大鼠脊髓血管周围间隙的超微结构及其在液体运输中的作用。应用光学显微镜、共聚焦显微镜和电子显微镜研究注入蛛网膜下腔的脑脊液示踪剂的分布。血管周围的空间被发现周围的小动脉和小静脉，但没有毛细血管，整个脊髓白色和灰质。它们含有成纤维细胞和胶原纤维，并与周围组织的细胞外空间连续。注射后5 min，在蛛网膜下腔、外周白色物质、血管周围空间、基底膜、周围组织的细胞外空间中观察到示踪剂，令人惊讶的是，在血管腔中观察到示踪剂，表明经血管清除。这些发现指出了CNS液体的未识别的流出途径，对健康和疾病状态下的容量调节具有潜在意义，而且在临床上用于检测血浆中CNS衍生的生物标志物、免疫应答和药物药代动力学。
Perivascular spaces play a pivotal role in the exchange between cerebrospinal and interstitial fluids, and in the clearance of waste in the CNS, yet their precise anatomical components are not well described. The aim of this study was to characterise the ultrastructure of perivascular spaces and their role in the transport of fluid, in the spinal cord of healthy rats, using transmission electron microscopy. The distribution of cerebrospinal fluid tracers injected into the subarachnoid space was studied using light, confocal and electron microscopy. Perivascular spaces were found around arterioles and venules, but not capillaries, throughout the spinal cord white and grey matter. They contained fibroblasts and collagen fibres, and were continuous with the extracellular spaces of the surrounding tissue. At 5 min post injection, tracers were seen in the subarachnoid space, the peripheral white matter, the perivascular spaces, basement membranes, extracellular spaces of the surrounding tissue, and surprisingly, in the lumen of blood vessels, suggesting trans-vascular clearance. These findings point out an unrecognised outflow pathway for CNS fluids, with potential implications for volume regulation in health and disease states, but also clinically for the detection of CNS-derived biomarkers in plasma, the immune response and drug pharmacokinetics.
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影响因子：
3.6
作者：
CIFUENTES, M;FERNANDEZLLEBREZ, P;RODRIGUEZ, EM
通讯作者：
RODRIGUEZ, EM
影响因子：
17.1
作者：
Hutter, Eliza;Boridy, Sebastien;Maysinger, Dusica
通讯作者：
Maysinger, Dusica
影响因子：
4.1
作者：
Chuang, Christine Y.;Degendorfer, Georg;Davies, Michael J.
通讯作者：
Davies, Michael J.
影响因子：
17.1
作者：
Iliff JJ;Wang M;Liao Y;Plogg BA;Peng W;Gundersen GA;Benveniste H;Vates GE;Deane R;Goldman SA;Nagelhus EA;Nedergaard M
通讯作者：
Nedergaard M
影响因子：
4.4
作者：
Jessen NA;Munk AS;Lundgaard I;Nedergaard M
通讯作者：
Nedergaard M