Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products.
Reflection and observation: cell-based screening failing to detect HBV in HUMSCs derived from HBV-infected mothers underscores the importance of more stringent donor eligibility to reduce risk of transmission of infectious diseases for stem cell-based medical products.
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反思和观察：基于细胞的筛查未能在源自 HBV 感染母亲的 HUMSC 中检测到 HBV，这凸显了更严格的捐赠者资格对于降低基于干细胞的传染病传播风险的重要性
DOI：
10.1186/s13287-018-0920-3
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发表时间：
2018-07-04
影响因子：
7.5
通讯作者：
Wang B
中科院分区：
文献类型：
--
作者：
Liu W;Xie Y;Gao T;Huang F;Wang L;Ding L;Wang W;Liu S;Dai J;Wang B
在细胞疗法中，传染病的传播对接受者构成了重大威胁。在这项研究中，我们研究了基于细胞的筛查是否可以检测从HBV感染供者分离的人脐带源性间充质干细胞（HUMSCs）中的乙型肝炎病毒（HBV），以了解HUMSCs对HBV感染的易感性。采用酶联免疫吸附法（ELISA）、荧光定量PCR法（FQ-PCR）和滴数PCR法（ddPCR）对健康和HBV感染供者的HUMSCs进行HBV检测。此外，使用FQ-PCR技术检测来自健康供体的HUMSCs与含有高滴度HBV的人血清孵育后的HBV DNA。ELISA、FQ-PCR、ddPCR均未检测到HBV抗原/抗体和DNA。与HBV感染血清孵育后，可以检测到HBV DNA，但低于检测试剂盒的有效效价。在HBV培养的HUMSCs中，HBV DNA水平随着每2天更换一次培养基逐渐下降，然后显著下降，传代后甚至检测不到。目前基于细胞的筛查方法无法在来自HBV感染供体的HUMSCs中检测到HBV，这表明在基于细胞的治疗中，更严格的供体资格对于降低传染病传播风险的重要性。为了解决供体资格确定中隐匿的HBV窗口期问题，我们建议供体在第一次血清学传染病筛查后3个月再进行一次HBV血清学检测。
In cell-based therapy, the transmission of communicable diseases imposes a substantial threat to recipients. In this study, we investigated whether cell-based screening could detect hepatitis B virus (HBV) in human umbilical cord-derived mesenchymal stem cells (HUMSCs) isolated from HBV-infected donors to understand the susceptibility of HUMSCs to HBV infection. HBV assay was performed in HUMSCs derived from healthy and HBV-infected donors with enzyme-linked immunosorbent assay (ELISA), fluorescence quantitative PCR (FQ-PCR) assay, and droplet digital PCR (ddPCR) assay. Further, HBV DNA was assayed in HUMSCs derived from healthy donors after incubation with human sera containing a high titer of HBV using FQ-PCR. HBV antigen/antibody and DNA failed to be detected using ELISA, FQ-PCR, and ddPCR. After incubation with HBV infection sera, HBV DNA could be detected, but below the valid titer of the assay kit. The HBV DNA levels in HBV-incubated HUMSCs gradually decreased with medium change every 2 days and then significantly decreased, not even detected after passage. The current cell-based screening methods could not detect HBV in HUMSCs derived from HBV-infected donors, indicating the importance of more stringent donor eligibility to reduce the risk of transmission of communicable diseases in cell-based therapy. To solve the problem of an occult HBV window period in donor eligibility determination, we recommend that the donors undergo another HBV serological test 3 months after the first serological communicable disease screening.
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影响因子：
5.9
作者：
Daley GQ;Hyun I;Apperley JF;Barker RA;Benvenisty N;Bredenoord AL;Breuer CK;Caulfield T;Cedars MI;Frey-Vasconcells J;Heslop HE;Jin Y;Lee RT;McCabe C;Munsie M;Murry CE;Piantadosi S;Rao M;Rooke HM;Sipp D;Studer L;Sugarman J;Takahashi M;Zimmerman M;Kimmelman J
通讯作者：
Kimmelman J
影响因子：
5.2
作者：
Romanov, YA;Svintsitskaya, VA;Smirnov, VN
通讯作者：
Smirnov, VN
影响因子：
4
作者：
Hsieh, Jui-Yu;Fu, Yu-Show;Wang, Hsei-Wei
通讯作者：
Wang, Hsei-Wei
影响因子：
4.2
作者：
RABER, IM;FRIEDMAN, HM
通讯作者：
FRIEDMAN, HM
影响因子：
5.2
作者：
in't Anker, PS;Scherjon, SA;Kanhai, HHH
通讯作者：
Kanhai, HHH