Masked Multiplexed Separations to Enhance Duty Cycle for Structures for Lossless Ion Manipulations.
Masked Multiplexed Separations to Enhance Duty Cycle for Structures for Lossless Ion Manipulations.
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DOI：
10.1021/acs.analchem.0c04799
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发表时间：
2021-04-13
影响因子：
7.4
通讯作者：
DeBord JD
中科院分区：
文献类型：
--
作者：
Clowers BH;Cabrera E;Anderson G;Deng L;Moser K;Van Aken G;DeBord JD
每个光谱一个离子包释放的实验范式严重阻碍了宽带离子迁移谱系统（例如漂移管和行波系统）的吞吐量。离子捕获略微缓解了这个问题，但是当移动到高分辨率，长路径长度系统时，占空比赤字被放大。因此，在保持峰值保真度的同时最大限度地提高吞吐量的新多路复用策略对于高分辨率IMS分离（例如SLIM和多通道技术）至关重要。目前，广泛适用的基于hadamard的离子复用反卷积策略仅限于狭窄的调制序列范围，并且不能充分最大化在扩展路径长度分离期间产生的离子信号。与之前依赖于峰值拾取或离散误差分类的Hadamard反褶积误差相比，掩模反褶积矩阵技术利用了Hadamard变换伪像反映关于中心主信号（即真实到达时间分布（ATD））的知识。通过随机诱导数学伪影，可以简单地通过相对于核心ATD的高度可变性来识别光谱伪影。重要的是要注意，使用掩模反卷积矩阵的减权方法对底层变换不做任何假设，并且适用于使用二进制序列的任何复用策略。除了证明检测到的离子总数增加了100倍之外，对来自5,6,7和8位伪随机序列的数据进行有效的反卷积扩展了SLIM平台的实用性和效率。
The experimental paradigm of one ion packet release per spectrum severely hinders throughput in broadband ion mobility spectrometry systems (e.g. drift tube and traveling wave systems). Ion trapping marginally mitigates this problem, but the duty cycle deficit is amplified when moving to high resolution, long pathlength systems. As a consequence, new multiplexing strategies that maximize throughput while preserving peak fidelity are essential for high resolution IMS separations (e.g. SLIM and multi-pass technologies). Currently, broadly applicable deconvolution strategies for Hadamard-based ion multiplexing are limited to a narrow range of modulation sequences and do not fully maximize the ion signal generated during separation across an extended path length. Compared to prior Hadamard deconvolution errors that rely upon peak picking or discrete error classification, the masked deconvolution matrix technique exploits the knowledge that Hadamard transform artifacts are reflected about the central, primary signal (i.e. the true arrival time distribution (ATD)). By randomly inducing mathematical artifacts it is possible to identify spectral artifacts simply by their high degree of variability relative to the core ATD. It is important to note that the deweighting approach using the masked deconvolution matrix does not make any assumptions about the underlying transform and is applicable to any multiplexing strategy employing binary sequences. In addition to demonstrating a 100-fold increase in the total number of ions detected, the effective deconvolution of data from 5, 6, 7, and 8-bit pseudo-random sequences expands the utility and efficiency of the SLIM platform.
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影响因子：
7.4
作者：
Poltash ML;McCabe JW;Shirzadeh M;Laganowsky A;Clowers BH;Russell DH
通讯作者：
Russell DH
影响因子：
7.4
作者：
KNORR, FJ;EATHERTON, RL;HILL, HH
通讯作者：
HILL, HH
影响因子：
3.5
作者：
Hanley, QS
通讯作者：
Hanley, QS
DOI：
10.1039/c7an00031f
发表时间：
2017-03-27
期刊：
The Analyst
影响因子：
--
作者：
Ibrahim YM;Hamid AM;Deng L;Garimella SV;Webb IK;Baker ES;Smith RD
通讯作者：
Smith RD
影响因子：
7.4
作者：
Li, Ailin;Nagy, Gabe;Conant, Christopher R.;Norheim, Randolph, V;Lee, Joon Yong;Giberson, Cameron;Hollerbach, Adam L.;Prabhakaran, Venkateshkumar;Attah, Isaac K.;Chouinard, Christopher D.;Prabhakaran, Aneesh;Smith, Richard D.;Ibrahim, Yehia M.;Garimella, Sandilya V. B.
通讯作者：
Garimella, Sandilya V. B.