A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities
A novel exopolysaccharide from deep-sea bacterium Zunongwangia profunda SM-A87: low-cost fermentation, moisture retention, and antioxidant activities
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来自深海细菌 Zunongwangia profunda SM-A87 的新型胞外多糖：低成本发酵、保湿和抗氧化活性
DOI：
10.1007/s00253-014-5839-8
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发表时间：
2014-05
影响因子：
5
通讯作者：
Bin-Bin Xie
中科院分区：
文献类型：
--
作者：
Qi-Long Qin;Bai-Cheng Zhou;Yu-Zhong Zhang;Bin-Bin Xie
许多海洋微生物可以分泌胞外多糖（EPS），其在生物技术中具有重要应用。我们从深海细菌 Zunongwangia profunda SM-A87 中纯化出一种新型 EPS，鉴定了其糖基组成和连接，并在之前的研究中将其产量优化至 8.9 g/l。为了降低发酵成本，开发了一种经济的发酵培养基，其中含有 60.9% 乳清、10 g/l 豆粕和 2.9% NaCl。该培养基分批发酵的EPS产量达到12.1±0.3g/l。进行补料分批发酵，EPS 产量为 17.2 ± 0.4 g/l，这是迄今为止报道的海洋细菌的最高 EPS 产量。提取的EPS表现出良好的流变特性、保湿能力和抗氧化活性。特别是，其保湿能力优于迄今为止报道的其他海洋细菌 EPS。 SM-A87 EPS 还表现出高抗氧化活性。这些结果表明 SM-A87 EPS 在生物技术方面具有广阔的潜力。
Many marine microorganisms can secrete exopolysaccharides (EPSs) which have important applications in biotechnology. We have purified a novel EPS from deep-sea bacterium Zunongwangia profunda SM-A87, identified its glycosyl composition and linkage, and optimized its production to 8.9 g/l in previous studies. To reduce the fermentation cost, an economical fermentation medium containing 60.9 % whey, 10 g/l soybean meal, and 2.9 % NaCl was developed. The EPS yield of batch fermentation in this medium reached 12.1 ± 0.3 g/l. Fed-batch fermentation was conducted and led to an EPS yield of 17.2 ± 0.4 g/l, which represents the highest EPS yield ever reported for a marine bacterium. The EPS was extracted and it displayed good rheological properties, moisture-retention ability, and antioxidant activity. Particularly, its moisture-retention ability is superior to that of other marine bacterial EPSs reported to date. SM-A87 EPS also showed high antioxidant activity. These results suggest that SM-A87 EPS has promising potentials in biotechnology.
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影响因子：
6.4
作者：
Liu L;Liu Y;Li J;Du G;Chen J
通讯作者：
Chen J
DOI：
10.1021/bk-1994-0573
发表时间：
1994-10
期刊：
No source information available
影响因子：
--
作者：
F. L. Buchholz;N. Peppas
通讯作者：
F. L. Buchholz;N. Peppas
DOI：
10.1016/j.scitotenv.2013.06.080
发表时间：
2013-10
期刊：
The Science of the total environment
影响因子：
--
作者：
A. Prazeres;F. Carvalho;J. Rivas;M. Patanita;J. Dôres
通讯作者：
A. Prazeres;F. Carvalho;J. Rivas;M. Patanita;J. Dôres
影响因子：
3.9
作者：
A. Diplock
通讯作者：
A. Diplock
影响因子：
3
作者：
Hyun Mi Kim;S. Paik;K. Ra;Kwang Bon Koo;J. Yun;J. Choi
通讯作者：
Hyun Mi Kim;S. Paik;K. Ra;Kwang Bon Koo;J. Yun;J. Choi