Inhibition of autophagy enhanced cobalt chloride‑induced apoptosis in rat alveolar type II epithelial cells.
Inhibition of autophagy enhanced cobalt chloride‑induced apoptosis in rat alveolar type II epithelial cells.
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DOI：
10.3892/mmr.2018.9209
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发表时间：
2018-08
影响因子：
3.4
通讯作者：
Han F
中科院分区：
文献类型：
--
作者：
Yu Y;Li W;Ren L;Yang C;Li D;Han X;Sun Y;Lv C;Han F
缺氧是一种可导致细胞凋亡和自噬的细胞应激。自噬和凋亡之间联系的分子机制仍不清楚，特别是在缺氧条件下。采用透射电镜、AO-PI染色、流式细胞术和westernblot等方法检测缺氧条件下自噬与凋亡的相互作用。大鼠肺泡II型上皮细胞RLE-6 TN细胞培养在一个长期的低氧环境建立氯化钴（II）。结果表明，缺氧条件下RLE-6 TN细胞发生自噬和凋亡。用自噬抑制剂3-甲基腺嘌呤处理RLE-6 TN细胞增加了活性氧的产生，线粒体损伤和缺氧诱导的凋亡。半胱天冬酶，特别是半胱天冬酶-9的表达增加，并可能参与了这些过程。这些数据表明，自噬的抑制增强细胞凋亡，通过介导的内源性途径。这些发现提供了重要的洞察自噬和凋亡串扰的分子机制。这可能为肺部疾病的监测、诊断和治疗提供新的见解。
Hypoxia is a type of cellular stress that may result in apoptosis and autophagy. The molecular mechanisms underlying the association between autophagy and apoptosis remain unclear, particularly in hypoxic conditions. Transmission electron microscope, AO-PI staining, flow cytometry and western blot were used to examine the crosstalk between autophagy and apoptosis in hypoxic conditions. Rat alveolar type II epithelial RLE-6TN cells were cultured in a long-term hypoxic environment established by cobalt (II) chloride. It was demonstrated that autophagy and apoptosis occurred in RLE-6TN cells under hypoxic conditions. Treatment of RLE-6TN cells with the autophagy inhibitor 3-methyladenine increased the generation of reactive oxygen species, mitochondrial damage and hypoxia-induced apoptosis. The expression of caspases, particularly caspase-9, increased and may have participated in these processes. The data indicated that the inhibition of autophagy enhanced apoptosis through the mitochondria-mediated intrinsic pathway. These findings provide important insight into the molecular mechanism of autophagy and apoptosis crosstalk. This may provide new insights into pulmonary disease surveillance, diagnosis and treatment.
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VHL肿瘤抑制剂：HIF的主调节器。
影响因子：
3.1
作者：
Haase VH
通讯作者：
Haase VH
影响因子：
2.5
作者：
Park, Junghyung;Min, Ju-Sik;Lee, Dong-Seok
通讯作者：
Lee, Dong-Seok
DOI：
10.1016/s0169-328x(98)00301-5
发表时间：
1999-01-22
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Badr, GA;Zhang, JZ;Ismail-Beigi, F
通讯作者：
Ismail-Beigi, F
影响因子：
5.3
作者：
Bellot, Gregory;Garcia-Medina, Raquel;Mazure, Nathalie M.
通讯作者：
Mazure, Nathalie M.
影响因子：
13.3
作者：
Grishchuk, Yulia;Ginet, Vanessa;Puyal, Julien
通讯作者：
Puyal, Julien