The cells and peripheral representation of sodium taste in mice.
The cells and peripheral representation of sodium taste in mice.
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作者：
哺乳动物的盐味可以引发两种不同的行为反应。一般来说，浓盐水溶液引起强烈的行为厌恶，而低浓度的NaCl通常是有吸引力的，特别是在钠耗尽后。值得注意的是，有吸引力的盐途径是选择性地响应钠和抑制阿米洛利，而厌恶的功能作为一个非选择性检测器的广泛的盐。由于阿米洛利是上皮钠通道（ENaC）的有效抑制剂，因此已提出ENaC作为盐味觉受体系统的组分起作用。在这里，我们研究了哺乳动物味觉系统中钠感应的基础。之前，我们发现五种基本味觉品质中的四种，甜，酸，苦和鲜味是由单独的味觉受体细胞（TRC）介导的，每个TRC都被调谐到一种单一的味觉形态，并被连接以引起刻板的行为反应。我们现在证明，钠传感也是由一个专门的人口TRCs介导的。这些味觉细胞表达上皮钠通道ENaC，并介导对NaCl的行为吸引。我们对TRC中缺乏ENaCα的小鼠进行了基因工程改造，并产生了表现出完全丧失盐吸引力和钠味觉反应的动物。总之，这些研究证实了所有五种基本味觉品质的独立细胞底物，并验证了ENaC对小鼠钠味觉的重要作用。
Salt taste in mammals can trigger two divergent behavioural responses. In general, concentrated saline solutions elicit robust behavioural aversion, while low concentrations of NaCl are typically attractive, particularly after sodium depletion. Notably, the attractive salt pathway is selectively responsive to sodium and inhibited by amiloride, while the aversive one functions as a non-selective detector for a wide range of salts. Since amiloride is a potent inhibitor of the epithelial sodium channel (ENaC), ENaC has been proposed to function as a component of the salt taste receptor system. Here, we examine the basis of sodium sensing in the mammalian taste system. Previously, we showed that four of the five basic taste qualities, sweet, sour, bitter and umami are mediated by separate taste receptor cells (TRC) each tuned to a single taste modality, and wired to elicit stereotypical behavioural responses. We now demonstrate that sodium sensing is also mediated by a dedicated population of TRCs. These taste cells express the epithelial sodium channel ENaC, and mediate behavioural attraction to NaCl. We genetically engineered mice lacking ENaCα in TRCs, and produced animals exhibiting a complete loss of salt attraction and sodium taste responses. Together, these studies substantiate independent cellular substrates for all five basic taste qualities, and validate the essential role of ENaC for sodium taste in mice.
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影响因子：
2.9
作者：
HELLEKANT, G;NINOMIYA, Y
通讯作者：
NINOMIYA, Y
影响因子：
56.9
作者：
HECK, GL;MIERSON, S;DESIMONE, JA
通讯作者：
DESIMONE, JA
影响因子：
3.3
作者：
Yoshida, R.;Horio, N.;Ninomiya, Y.
通讯作者：
Ninomiya, Y.
影响因子：
64.8
作者：
Nelson, G;Chandrashekar, J;Zuker, CS
通讯作者：
Zuker, CS
DOI：
10.1136/bmj.b4567
发表时间：
2009-11-24
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
Strazzullo P;D'Elia L;Kandala NB;Cappuccio FP
通讯作者：
Cappuccio FP