Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.
Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.
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DOI：
10.1016/j.vaccine.2011.12.134
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发表时间：
2012-02-14
期刊：
影响因子：
5.5
通讯作者：
Lukashevich, Igor S.
中科院分区：
文献类型：
--
作者：
Goicochea, Marco A.;Zapata, Juan C.;Bryant, Joseph;Davis, Harry;Salvato, Maria S.;Lukashevich, Igor S.
Lassa fever (LF) is one of the most prevalent viral hemorrhagic fevers in West Africa responsible for thousands of deaths annually. The BSL-4 containment requirement and lack of small animal model to evaluate Lassa virus (LASV)-specific cell-mediated immunity (CMI) complicate development of effective LF vaccines. Here we have described a CBA/J-ML29 model allowing evaluation of LASV-specific CMI responses in mice. This model is based on Mopeia virus reassortant clone ML29, an attractive immunogenic surrogate for LASV. A single intraperitoneal (i.p.) immunization of CBA/J mice with ML29 protected animals against a lethal homologous intracerebral (i.c.) challenge with 588 LD50. The ML29-immunized mice displayed negligible levels of LASV-specific antibody titers, but LASV-specific CMI responses were detectable early and peaked on day 8–10 after immunization. A T cell cytotoxicity assay in vivo showed a correlation between LASV-specific cytotoxicity and the timing of protection induced by the ML29 immunization. Notably, CBA/J mice that received CD8+ T cell-depleted splenocytes from ML29-immunized donors all succumbed to a lethal i.c. challenge, demonstrating that CD8+ T cells are critical in protection. The CBA/J-ML29 model can be useful immunological tool for the preliminary evaluation of immunogenicity and efficacy of vaccine candidates against LASV outside of BSL-4 containment facilities.
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表达拉沙病毒 GP1 和 GP2 糖蛋白的黄热病 17D 载体疫苗可为豚鼠提供针对致命疾病的保护。
影响因子：
5.5
作者：
Jiang, Xiaohong;Dalebout, Tim J.;Bredenbeek, Peter J.;Carrion, Ricardo, Jr.;Brasky, Kathleen;Patterson, Jean;Goicochea, Marco;Bryant, Joseph;Salvato, Maria S.;Lukashevich, Igor S.
通讯作者：
Lukashevich, Igor S.
影响因子：
5.5
作者：
Lukashevich IS;Carrion R Jr;Salvato MS;Mansfield K;Brasky K;Zapata J;Cairo C;Goicochea M;Hoosien GE;Ticer A;Bryant J;Davis H;Hammamieh R;Mayda M;Jett M;Patterson J
通讯作者：
Patterson J