Dependence of UV-B-induced camptothecin production on nitrate reductase-mediated nitric oxide signaling in Camptotheca acuminata suspension cell cultures
Dependence of UV-B-induced camptothecin production on nitrate reductase-mediated nitric oxide signaling in Camptotheca acuminata suspension cell cultures
复制标题
喜树悬浮细胞培养物中 UV-B 诱导的喜树碱产生对硝酸还原酶介导的一氧化氮信号传导的依赖性
DOI：
10.1007/s11240-014-0479-3
复制
发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Xu Maojun
中科院分区：
文献类型：
--
作者：
Ruan Jiazhao;Zhang Jinjie;Li Mengya;Zhu Yun;Sun Lina;Jin Haihong;Su Hu;Xu Maojun
UV-B辐照诱导植物细胞产生次生代谢物。然而，uv - b诱导次生代谢物产生的机制在很大程度上仍然未知。在这里，我们报道了UV-B处理刺激了喜树细胞中一氧化氮（NO）的产生和喜树碱（CPT）的产生。为了研究UV-B触发NO的来源以及NO在UV-B诱导CPT生成中的作用，我们检测了细胞中硝酸盐还原酶（NR）和NO合成酶（NOS）活性对UV-B暴露的反应，并研究了NR和NOS抑制剂对UV-B处理细胞中CPT生成的影响。结果表明，UV-B照射可增强细胞内NR活性。NR抑制剂钨酸酯和冈田酸预处理不仅能抑制uv - b诱导的NR活性，还能抑制uv - b诱导的细胞NO生成和CPT生成。相比之下，UV-B照射对细胞的NOS活性没有影响，NOS抑制剂处理也没有抑制UV-B诱导的CAT生成。综上所述，研究结果表明NR活性对于uv - b触发的NO生成至关重要，并且NR介导的NO信号通路参与了uv - b诱导的CPT生成。
UV-B irradiation induced production of secondary metabolites in plant cells. However, the mechanisms of UV-B-induced secondary metabolite production remained largely unknown. Here we report that UV-B treatment stimulated nitric oxide (NO) generation and camptothecin (CPT) production in Camptotheca acuminata cells. To investigate the origin of the UV-B-triggered NO and the role of NO in UV-B-induced CPT production, we assayed the responses of nitrate reductase (NR) and NO synthase (NOS) activities of the cells to UV-B exposure and examined the effects of NR and NOS inhibitors on CPT production in UV-B-treated cells. The data showed that UV-B irradiation enhanced NR activities in the cells. Pretreatment with NR inhibitors tungstate and okadaic acid not only suppressed the UV-B-triggered NR activities but also inhibited the UV-B-induced NO generation and CPT production in the cells. In contrast, UV-B irradiation had no effects on NOS activity of the cells and treatment of NOS inhibitor did not suppress UV-B-induced CAT production. Together, the results demonstrated that NR activity was essential for UV-B-triggered NO generation and that NR-mediated NO signaling was involved in UV-B-induced CPT production in C. acuminata cells.
登录
查看更多内容
DOI：
10.1007/s11427-006-2010-5
发表时间：
2006-08
期刊：
Science in China Series C: Life Sciences
影响因子：
--
作者：
Maojun Xu;Jufang Dong;Mu-yuan Zhu
通讯作者：
Maojun Xu;Jufang Dong;Mu-yuan Zhu
影响因子：
--
作者：
M. Murphy;E. Noack
通讯作者：
M. Murphy;E. Noack
影响因子：
7.4
作者：
DEAN, JV;HARPER, JE
通讯作者：
HARPER, JE
影响因子：
1.2
作者：
A. Yamamoto;S. Katou;H. Yoshioka;N. Doke;K. Kawakita
通讯作者：
A. Yamamoto;S. Katou;H. Yoshioka;N. Doke;K. Kawakita
DOI：
10.1111/j.1365-3040.2007.01761.x
发表时间：
2008-05
期刊：
Plant, cell & environment
影响因子：
--
作者：
I. Wilson;S. Neill;J. Hancock
通讯作者：
I. Wilson;S. Neill;J. Hancock