Optix defines a neuroepithelial compartment in the optic lobe of the Drosophila brain.
Optix defines a neuroepithelial compartment in the optic lobe of the Drosophila brain.
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DOI：
10.1186/1749-8104-9-18
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发表时间：
2014-07-29
影响因子：
3.6
通讯作者：
Brand AH
中科院分区：
文献类型：
--
作者：
Gold KS;Brand AH
在大脑早期发育过程中，神经祖细胞组织成神经上皮层，在生长过程中维持组织的完整性。神经上皮的黏附和模式形成对于有序增殖和神经命运特化至关重要。神经上皮通过转录因子和信号分子的表达实现区域化，从而形成不同的发育区域，并最终形成功能区域。
我们发现Six3/6家族同源物Optix是果蝇大脑中神经上皮维持和模式形成的关键调节因子。Six3和Six6是哺乳动物眼睛和前脑发育所必需的，人类中的突变与严重的眼睛和大脑畸形有关。在果蝇中，Optix在幼虫视叶的一个界限分明的区域表达，其表达与转录因子Vsx1的表达相互对应。Optix功能获得和缺失会影响神经上皮的黏附、完整性和极性。我们发现了与转录因子表达区域相对应的受限细胞谱系边界。
我们提出视叶通过Optix和Vsx1的表达实现分区化。我们的研究结果为大脑一个复杂区域的空间模式形成提供了见解，并提示了视觉系统发育的一个进化上保守的原则。
During early brain development, the organisation of neural progenitors into a neuroepithelial sheet maintains tissue integrity during growth. Neuroepithelial cohesion and patterning is essential for orderly proliferation and neural fate specification. Neuroepithelia are regionalised by the expression of transcription factors and signalling molecules, resulting in the formation of distinct developmental, and ultimately functional, domains. We have discovered that the Six3/6 family orthologue Optix is an essential regulator of neuroepithelial maintenance and patterning in the Drosophila brain. Six3 and Six6 are required for mammalian eye and forebrain development, and mutations in humans are associated with severe eye and brain malformation. In Drosophila, Optix is expressed in a sharply defined region of the larval optic lobe, and its expression is reciprocal to that of the transcription factor Vsx1. Optix gain- and loss-of-function affects neuroepithelial adhesion, integrity and polarity. We find restricted cell lineage boundaries that correspond to transcription factor expression domains. We propose that the optic lobe is compartmentalised by expression of Optix and Vsx1. Our findings provide insight into the spatial patterning of a complex region of the brain, and suggest an evolutionarily conserved principle of visual system development.
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影响因子：
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