Characterization of microRNA expression profiles in Leishmania-infected human phagocytes.
Characterization of microRNA expression profiles in Leishmania-infected human phagocytes.
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DOI：
10.1111/pim.12156
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发表时间：
2015-01
影响因子：
2.2
通讯作者：
McDowell MA
中科院分区：
文献类型：
--
作者：
Geraci NS;Tan JC;McDowell MA
Leishmania are intracellular protozoa that influence host immune responses eliciting parasite species specific pathologies. MicroRNAs (miRNA) are short single stranded ribonucleic acids that complement gene transcripts to block protein translation and have been shown to regulate immune system molecular mechanisms. Human monocyte derived dendritic cells (DC) and macrophages (MP) were infected in vitro with Leishmania major or Leishmania donovani parasites. Small RNAs were isolated from total RNA and sequenced to identify mature miRNAs associated with leishmanial infections. Normalized sequence read count profiles revealed a global down-regulation in miRNA expression among host cells following infection. Most identified miRNAs were expressed at higher levels in L. donovani infected cells relative to L. major infected cells. Pathway enrichments using in silico predicted gene targets of differentially expressed miRNAs showed evidence of potentially universal MAP kinase signaling pathway effects. Whereas JAK-STAT and TGF-β signaling pathways were more highly enriched using targets of miRNAs up-regulated in L. donovani infected cells. These data provide evidence in support of a selective influence on host cell miRNA expression and regulation in response to differential Leishmania infections.
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