Cartilage reconstruction in head and neck surgery: comparison of resorbable polymer scaffolds for tissue engineering of human septal cartilage.
Cartilage reconstruction in head and neck surgery: comparison of resorbable polymer scaffolds for tissue engineering of human septal cartilage.
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头颈手术中的软骨重建：用于人体间隔软骨组织工程的可吸收聚合物支架的比较。
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
M. Sittinger
中科院分区：
文献类型：
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作者：
N. Rotter;J. Aigner;A. Naumann;H. Planck;C. Hammer;G. Burmester;M. Sittinger
新的细胞培养技术提高了在组织工程的帮助下在体外创造软骨的可能性。在这项研究中，我们比较了两种可吸收的非织造细胞支架，聚乙醇酸/聚-L-乳酸（PGA/PLLA）（90/10）共聚物（Ethisorb）和纯PLLA（V 7-2），它们在软骨重建方面具有不同的降解特性。软骨细胞从人中隔软骨酶分离。将单细胞重悬于琼脂糖中并转移到聚合物支架中以产生机械稳定性并保留软骨细胞特异性表型。然后将细胞-聚合物构建体在灌注培养中保持1周，然后皮下移植到胸腺增生性裸鼠中。6、12和24周后，对标本进行染色，并对胶原（azan染色）、蛋白多糖（Alcian蓝染色）和钙化区域（von Kossa染色）的存在进行组织化学分析。此外，不同类型的胶原蛋白（胶原蛋白I型，这是在大多数组织中发现的，但不是在透明软骨基质;和胶原蛋白II型，这是软骨特异性）进行了化学鉴别的间接免疫过氧化物酶技术。血管向内生长通过因子VIII抗体进行研究，因子VIII抗体是一种内皮标志物。使用Photoshop软件对几种基质组分进行定量。发现两种非织造结构之间关于基质合成和基质质量以及血管向内生长的显著差异。Ethisorb在体外降解时间约为3周，移植后24周，I型和II型胶原蛋白的量与正常人中隔软骨无显著差异。含有血管的薄纤维组织层包裹着移植物。V7 -2构建体，即使在移植后24周也没有表现出强烈的降解迹象，含有明显更少量的软骨特异性基质成分。同时，移植物的中心部分也有血管向内生长。总之，降解时间短的聚合物支架是开发软骨基质产品的合适材料，而较长的稳定性似乎会抑制基质合成。因此，当使用适当的非织造物时，人软骨的体外工程化可产生软骨样组织。因此，这种方法可能是理想的软骨替代方法，没有感染的风险，并有可能重建具有不同构型的大缺损。
New cell culture techniques raise the possibility of creating cartilage in vitro with the help of tissue engineering. In this study, we compared two resorbable nonwoven cell scaffolds, a polyglycolic acid/poly-L-lactic acid (PGA/PLLA) (90/10) copolymer (Ethisorb) and pure PLLA (V 7-2), with different degradation characteristics in their aptitude for cartilage reconstruction. Chondrocytes were isolated enzymatically from human septal cartilage. The single cells were resuspended in agarose and transferred into the polymer scaffolds to create mechanical stability and retain the chondrocyte-specific phenotype. The cell-polymer constructs were then kept in perfusion culture for 1 week prior to subcutaneous transplantation into thymusaplastic nude mice. After 6, 12, and 24 weeks, the specimens were explanted and analyzed histochemically on the presence of collagen (azan staining), proteoglycans (Alcian blue staining), and calcification areas (von Kossa staining). Furthermore, different collagen types (collagen type I, which is found in most tissues, but not in hyaline cartilage matrix; and collagen type II, which is cartilage specific) were differentiated immunohistochemically by the indirect immunoperoxidase technique. Vascular ingrowth was investigated by a factor VIII antibody, which is a endothelial marker. Quantification of several matrix components was performed using the software Photoshop. Significant differences were found between both nonwoven structures concerning matrix synthesis and matrix quality as well as vascular ingrowth. Ethisorb, with a degradation time of approximately 3 weeks in vitro, showed no significant differences from normal human septal cartilage in the amount of collagen types I and II 24 weeks after transplantation. Thin fibrous tissue layers containing blood vessels encapsulated the transplants. V 7-2 constructs, which did not show strong signs of degradation even 24 weeks after transplantation, contained remarkably smaller amounts of cartilage-specific matrix components. At the same time, there was vascular ingrowth even in central parts of the transplants. In conclusion, polymer scaffolds with a short degradation time are suitable materials for the development of cartilage matrix products, while longer stability seems to inhibit matrix synthesis. Thus, in vitro engineering of human cartilage can result in a cartilage-like tissue when appropriate nonwovens are used. Therefore, this method could be the ideal cartilage replacement method without the risk of infection and with the possibility of reconstructing large defects with different configurations.
软骨基质的化学稳定。
DOI：
10.1006/jsre.1994.1047
发表时间：
1994
期刊：
The Journal of surgical research
影响因子：
--
作者：
Kahn,A;Pottenger,LA;Albertini,JG;Taitz,AD;Thonar,EJ
通讯作者：
Thonar,EJ
影响因子：
56.9
作者：
MOSES, MA;SUDHALTER, J;LANGER, R
通讯作者：
LANGER, R