Synthesis and Characterization of Thiolate-Protected Gold Nanoparticles of Controlled Diameter
Synthesis and Characterization of Thiolate-Protected Gold Nanoparticles of Controlled Diameter
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受硫醇保护的可控直径金纳米粒子的合成与表征
DOI：
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发表时间：
2019
影响因子：
3.7
通讯作者：
Y. Levi
中科院分区：
文献类型：
--
作者：
L. Shaltiel;Asaf Shemesh;U. Raviv;Y. Barenholz;Y. Levi
金纳米颗粒（AuNPs）由于其独特的光学和电子特性以及广泛的应用而成为许多研究的焦点。然而，合成具有特定大小选择的稳定且均匀的aunp仍然是一个挑战。在这项研究中，我们描述了一种直接合成方法来生产稳定的单分散水溶性AuNPs，其直径严格控制在1.7-2.4 nm范围内。我们仅通过改变合成过程中氢氧化钠（NaOH）的浓度来控制尺寸。凝胶电泳、透射电子显微镜（TEM）和溶液x射线散射显示，AuNPs具有狭窄的尺寸分布。我们进一步发现，不同大小的aunp在TEM显微镜下可以清晰区分，为双靶点标记铺平了道路。AuNPs对DNA和蛋白质的反应性也得到了证实。我们利用这种反应性来标记嵌入在抗癌纳米药物Doxil膜中的尾部锚定蛋白，作为一种靶向…
Gold nanoparticles (AuNPs) have been the focus of many studies owing to their unique optical and electronic properties and versatile applications. However, synthesis of stable and homogeneous AuNPs with a particular choice of size is still a challenge. In this study we describe a direct synthesis approach to produce stable and monodisperse water-soluble AuNPs with a tightly controlled diameter in the 1.7–2.4 nm range. We controlled the size by changing only the sodium hydroxide (NaOH) concentration in the synthesis. Gel electrophoresis, transmission electron microscopy (TEM), and solution X-ray scattering showed that the AuNPs had narrow size-distributions. We further showed that AuNPs of the different sizes were clearly distinguishable in TEM micrographs, paving the way to dual-target labeling. The reactivity of the AuNPs toward DNA and proteins was also demonstrated. We utilized this reactivity to label tail-anchored proteins embedded in the membrane of the anticancer nanodrug Doxil as a means to target...
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影响因子：
15
作者：
Brinas, Raymond P.;Hu, Minghui;Hainfeld, James F.
通讯作者：
Hainfeld, James F.
影响因子：
15
作者：
Ackerson, CJ;Jadzinsky, PD;Kornberg, RD
通讯作者：
Kornberg, RD
影响因子：
4.7
作者：
Ackerson, Christopher J.;Jadzinsky, Pablo D.;Sexton, Jonathan Z.;Bushnell, David A.;Kornberg, Roger D.
通讯作者：
Kornberg, Roger D.
影响因子：
15
作者：
Ackerson, CJ;Jadzinsky, PD;Kornberg, RD
通讯作者：
Kornberg, RD
影响因子：
10.8
作者：
Azubel, Maia;Kornberg, Roger D.
通讯作者：
Kornberg, Roger D.