Contribution of Kupffer cells to liposome accumulation in the liver.
Contribution of Kupffer cells to liposome accumulation in the liver.
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DOI：
10.1016/j.colsurfb.2017.07.014
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发表时间：
2017-10-01
期刊：
影响因子：
--
通讯作者：
Wolfram J
中科院分区：
文献类型：
--
作者：
Samuelsson E;Shen H;Blanco E;Ferrari M;Wolfram J
肝脏是系统注射纳米粒局部特异性输送的主要障碍，因为高达90%的剂量通常被这个器官捕获。库普弗细胞被认为是导致纳米颗粒在肝脏中积聚的主要细胞成分。这些滞留的巨噬细胞构成单核巨噬细胞系统的一部分，该系统识别并吞噬循环系统中的异物。在这项研究中，我们比较了两种减少纳米颗粒在肝脏中积累的策略，以调查库普弗细胞的具体贡献。具体地说，我们对聚乙二醇化和枯否细胞耗竭减少脂质体在肝脏中堆积的能力进行了比较。聚乙二醇化可减少纳米颗粒与所有类型细胞的相互作用，并可作为一种对照，以阐明特定细胞群在肝脏聚集中的作用。结果表明，与枯否细胞耗尽相比，脂质体聚乙二醇化是避免肝脏摄取的一种更有效的策略，这表明纳米粒子与肝脏中其他细胞的相互作用也可能起到作用。这项研究强调需要更全面地了解调节纳米颗粒在肝脏中积累的因素，并探索微环境调节策略以减少该器官中纳米颗粒与细胞的相互作用。
The liver is a major barrier for site-specific delivery of systemically injected nanoparticles, as up to 90% of the dose is usually captured by this organ. Kupffer cells are thought to be the main cellular component responsible for nanoparticle accumulation in the liver. These resident macrophages form part of the mononuclear phagocyte system, which recognizes and engulfs foreign bodies in the circulatory system. In this study, we have compared two strategies for reducing nanoparticle accumulation in the liver, in order to investigate the specific contribution of Kupffer cells. Specifically, we have performed a comparison of the capability of pegylation and Kupffer cell depletion to reduce liposome accumulation in the liver. Pegylation reduces nanoparticle interactions with all types of cells and can serve as a control for elucidating the role of specific cell populations in liver accumulation. The results indicate that liposome pegylation is a more effective strategy for avoiding liver uptake compared to depletion of Kupffer cells, suggesting that nanoparticle interactions with other cells in the liver may also play a contributing role. This study highlights the need for a more complete understanding of factors that mediate nanoparticle accumulation in the liver and for the exploration of microenvironmental modulation strategies for reducing nanoparticle-cell interactions in this organ.
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影响因子：
41.2
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Chan, Warren C. W.
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影响因子：
17.4
作者：
Gustafson HH;Holt-Casper D;Grainger DW;Ghandehari H
通讯作者：
Ghandehari H
影响因子：
5.6
作者：
Sercombe L;Veerati T;Moheimani F;Wu SY;Sood AK;Hua S
通讯作者：
Hua S