Exo70 is an independent prognostic factor in colon cancer.
Exo70 is an independent prognostic factor in colon cancer.
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Exo70是结肠癌的独立预后因素
DOI：
10.1038/s41598-017-05308-x
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发表时间：
2017-07-11
影响因子：
4.6
通讯作者：
Hu T
中科院分区：
文献类型：
--
作者：
Xiao L;Zheng K;Lv X;Hou J;Xu L;Zhao Y;Song F;Fan Y;Cao H;Zhang W;Hong X;Zhan YY;Hu T
Exo 70是胞吐复合物的关键组分，在人类癌症进展中发挥着超越胞吐的重要作用。然而，Exo 70的表达及其对结肠癌患者的预后价值迄今尚未得到很好的研究。在这项研究中，我们观察到13例结肠癌组织中有11例的Exo 70 mRNA和蛋白水平上调，与正常组织相比，这是通过包含89对结肠癌组织和匹配的相邻正常组织的组织芯片中的免疫组织化学分析来验证的。Exo 70表达与肿瘤大小、浸润深度、TNM分期及远处转移呈正相关。Kaplan-Meier生存分析显示，具有较高Exo 70表达的结肠癌患者比具有较低Exo 70表达的患者具有较差的临床结局。多因素考克斯回归分析显示，Exo 70、年龄和远处转移是影响结肠癌患者生存率的独立预后因素。通过结肠癌细胞中Exo 70的获得和丧失，我们发现Exo 70可以增强结肠癌细胞的迁移能力。综上所述，我们的研究表明，Exo 70可能是一个有前途的负性预后因子和潜在的治疗靶点结肠癌。
Exo70, a key component of the Exocyst complex, plays important roles in human cancer progression beyond exocytosis. However, the expression of Exo70 and its prognostic value for patients with colon cancer has not been well investigated to date. In this study, we observed that the mRNA and protein levels of Exo70 were upregulated in 11 of 13 colon cancer tissues, compared with their normal counterparts, which was validated by immunohistochemical analysis in a tissue microarray containing 89 pairs of colon cancer tissues and the matched adjacent normal tissues. Statistical analysis revealed that Exo70 expression is positively correlated with tumor size, invasion depth, TNM stage and distant metastasis. Kaplan-Meier survival analysis showed that colon cancer patients with higher Exo70 expression have a poorer clinical outcome than those with lower Exo70 expression. Multivariate Cox regression analysis revealed that Exo70, age and distant metastasis were there independent prognostic factors for overall survival rate of colon cancer patients. Through gain- and loss of Exo70 in colon cancer cells, we found that Exo70 could enhance the migration ability of colon cancer cells. Taken together, our studies revealed that Exo70 might be a promising negative prognostic factor and a potential therapeutic target for colon cancer.
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DOI：
10.1073/pnas.1521248112
发表时间：
2016-01-05
影响因子：
11.1
作者：
Zhang, Chunhua;Brown, Michelle Q.;Raikhel, Natasha V.
通讯作者：
Raikhel, Natasha V.
影响因子：
9.2
作者：
Liu, Jianglan;Zhao, Yuting;Sun, Yujie;He, Bing;Yang, Changsong;Svitkina, Tatyana;Goldman, Yale E.;Guo, Wei
通讯作者：
Guo, Wei
影响因子：
50.5
作者：
Hashim, D.;Boffetta, P.;Negri, E.
通讯作者：
Negri, E.
影响因子：
16.8
作者：
Yamashita, Masami;Kurokawa, Kazuo;Fukai, Shuya
通讯作者：
Fukai, Shuya
影响因子：
--
作者：
Mukherjee, Debarati;Sen, Arpita;Aguilar, R Claudio
通讯作者：
Aguilar, R Claudio