Pathologic changes in mice induced by subtilase cytotoxin, a potent new Escherichia coli AB5 toxin that targets the endoplasmic reticulum.
Pathologic changes in mice induced by subtilase cytotoxin, a potent new Escherichia coli AB5 toxin that targets the endoplasmic reticulum.
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枯草杆菌酶细胞毒素（一种针对内质网的新型强效大肠杆菌 AB5 毒素）诱导小鼠发生病理变化。
DOI：
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发表时间：
2007
影响因子：
6.4
通讯作者：
A. Paton
中科院分区：
文献类型：
--
作者：
Hui Wang;J. Paton;A. Paton
枯草杆菌酶细胞毒素（Subtilase cytotoxin，SubAB）是近年来发现的AB（5）细胞毒素家族的原型，由滋贺菌（Stiga coli，STEC）的某些菌株产生。催化A亚基是高度特异性的枯草杆菌酶样丝氨酸蛋白酶，其切割内质网伴侣BiP。这种毒素对老鼠是致命的，但它引起的病理学却知之甚少。在这里，我们表明，腹腔注射SubAB导致小鼠微血管病性溶血性贫血，血小板减少症和肾功能损害-典型的滋贺毒素诱导的溶血性尿毒综合征的特征。亚AB引起广泛的微血管血栓形成和其他组织学损害的大脑，肾脏和肝脏，以及戏剧性的脾萎缩。外周血白细胞水平在24小时增加，也有显着的中性粒细胞浸润的肝脏，肾脏和脾脏和毒素诱导的细胞凋亡在这些网站。这些发现提高了SubAB直接导致感染产生滋贺毒素和SubAB的STEC菌株的人类的病理的可能性。
Subtilase cytotoxin (SubAB) is the prototype of a recently discovered AB(5) cytotoxin family produced by certain strains of Shiga toxigenic Escherichia coli (STEC). The catalytic A subunit is a highly specific subtilase-like serine protease that cleaves the endoplasmic reticulum chaperone BiP. The toxin is lethal for mice, but the pathology it induces is poorly understood. Here, we show that intraperitoneal injection of SubAB causes microangiopathic hemolytic anemia, thrombocytopenia, and renal impairment in mice--characteristics typical of Shiga toxin-induced hemolytic uremic syndrome. SubAB caused extensive microvascular thrombosis and other histologic damage in the brain, kidneys, and liver, as well as dramatic splenic atrophy. Peripheral blood leukocyte levels were increased at 24 h; there was also significant neutrophil infiltration in the liver, kidneys, and spleen and toxin-induced apoptosis at these sites. These findings raise the possibility that SubAB directly contributes to pathology in humans infected with strains of STEC that produce both Shiga toxin and SubAB.
影响因子：
4.8
作者：
Lee, AS
通讯作者：
Lee, AS
DOI：
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发表时间：
2004-10
期刊：
The Mount Sinai journal of medicine, New York
影响因子：
--
作者：
L. Hendershot
通讯作者：
L. Hendershot