Multiple functional P2X and P2Y receptors in the luminal and basolateral membranes of pancreatic duct cells.
Multiple functional P2X and P2Y receptors in the luminal and basolateral membranes of pancreatic duct cells.
复制标题
胰管细胞腔膜和基底外侧膜中的多功能 P2X 和 P2Y 受体。
DOI：
10.1152/ajpcell.1999.277.2.c205
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Muallem,S
中科院分区：
文献类型：
--
作者：
Luo,X;Zheng,W;Yan,M;Lee,MG;Muallem,S
胰管基底膜和管腔膜中的嘌呤能受体在胰管分泌过程中通过反馈机制协调两层膜的转运活动。本研究的目的是鉴定和定位大鼠胰管中功能P2受体（P2R）。由于克隆的P2R缺乏选择性激动剂和/或拮抗剂，因此需要使用分子和功能方法来表征导管P2R。在分子研究中，RNA是从胰腺小叶内管的微解剖中制备的，并被证明不含淀粉酶和内皮一氧化氮合酶的mRNA（分别是腺泡细胞和内皮细胞的标志物）。描述了一种新的程序，以获得适合电生理研究的单管细胞的富集制备。P2R的定位是通过检测各种P2R激动剂对微灌注小叶内导管细胞内Ca2+浓度（[Ca2+]i）的影响来实现的。RT-PCR分析显示P2R在胰管中有6个亚型的表达：3个P2YR亚型和3个P2XR亚型。各种核苷酸激活Cl -电流以及这些核苷酸激活的受体与G蛋白的偶联证实了导管细胞中多个P2R的表达。测量[Ca2+]iin微灌注的小叶内管提示P2X1R， P2X4R，可能是P2X7R，以及尚未确定的P2YR，可能是P2Y1R在基底外侧膜中的表达。P2Y2R、P2Y4R和P2X7R在管腔膜中表达。在一种细胞类型中前所未有地表达如此多种P2R，其中许多能够激活Cl -通道，表明这些受体可能在胰管细胞功能中起重要作用。
Purinergic receptors in the basolateral and luminal membranes of the pancreatic duct can act by a feedback mechanism to coordinate transport activity in the two membranes during ductal secretion. The goal of the present work was to identify and localize the functional P2 receptors (P2R) in the rat pancreatic duct. The lack of selective agonists and/or antagonists for any of the cloned P2R dictated the use of molecular and functional approaches to the characterization of ductal P2R. For the molecular studies, RNA was prepared from microdissected pancreatic intralobular ducts and was shown to be free of mRNA for amylase and endothelial nitric oxide synthase (markers for acinar and endothelial cells, respectively). A new procedure is described to obtain an enriched preparation of single duct cells suitable for electrophysiological studies. Localization of P2R was achieved by testing the effect of various P2R agonists on intracellular Ca2+concentration ([Ca2+]i) of microperfused intralobular ducts. RT-PCR analysis suggested the expression of six subtypes of P2R in the pancreatic duct: three P2YR and three P2XR. Activation of Cl−current by various nucleotides and coupling of the receptors activated by these nucleotides to G proteins confirmed the expression of multiple P2R in duct cells. Measurement of [Ca2+]iin microperfused intralobular ducts suggested the expression of P2X1R, P2X4R, probably P2X7R, and as yet unidentified P2YR, possibly P2Y1R, in the basolateral membrane. Expression of P2Y2R, P2Y4R, and P2X7R was found in the luminal membrane. The unprecedented expression of such a variety of P2R in one cell type, many capable of activating Cl−channels, suggests that these receptors may have an important role in pancreatic duct cell function.
登录
查看更多内容
在用 [Ca2+]i 动员激动剂刺激胰腺腺泡细胞期间，Gβγ 转导 [Ca2+]i 振荡，Gαq 产生持续反应*
影响因子：
4.8
作者：
W. Zeng;Xin Xu;S. Muallem
通讯作者：
S. Muallem
与 P2Y5 受体密切相关的人类七螺旋受体的克隆。
影响因子：
3.1
作者：
R. Janssens;J. Boeynaems;M. Godart;D. Communi
通讯作者：
D. Communi
大鼠颌下导管细胞上存在代谢型和离子型嘌呤能受体。
影响因子：
--
作者：
H. Amsallem;M. Métioui;A. Vandenabeele;A. Elyamani;A. Moran;J. Dehaye
通讯作者：
J. Dehaye
6H1孤儿受体，声称是p2y5受体，不介导核苷酸促进的第二信使反应。
影响因子：
3.1
作者：
Li,Q;Schachter,JB;Harden,TK;Nicholas,RA
通讯作者：
Nicholas,RA
影响因子：
5.8
作者：
Hootman,SR;Zukerman,J;Kovalcik,SA
通讯作者：
Kovalcik,SA