Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda.
Investigating the molecular mechanisms of organophosphate and pyrethroid resistance in the fall armyworm Spodoptera frugiperda.
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DOI：
10.1371/journal.pone.0062268
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Bass C
中科院分区：
文献类型：
--
作者：
Carvalho RA;Omoto C;Field LM;Williamson MS;Bass C
秋粘虫是一种重要的经济害虫，在美洲所有玉米种植区都有发生。为控制其而大量使用化学农药导致了抗性种群的选择，然而，迄今为止，抗性背后的分子机制尚未得到表征。本研究采用分子和基因组学方法研究了在巴西采集的两株frugiperda菌株对毒死蜱（OP菌株）和高效氯氟氰菊酯（PYR菌株）的抗性机制。为了研究靶位不敏感的可能作用，我们扩增了编码有机磷（乙酰胆碱酯酶，AChE）和拟除虫菊酯（电压门控钠通道，VGSC）靶位蛋白的基因。对S. frugiperda ace-1基因进行测序，发现OP菌株与敏感参考菌株（SUS）相比有几个核苷酸变化。这导致了A201S、G227A和F290V三个氨基酸的取代，这些氨基酸之前都被证明在其他几种昆虫中具有有机磷抗性。对PYR菌株中编码VGSC的基因进行测序，发现了导致三个氨基酸替换的突变，T929I， L932F和L1014F，所有这些突变先前都被证明在几种节肢动物物种中具有敲低/超级敲低型抗性。为了研究代谢解毒在OP和PYR染色抗性表型中的可能作用，我们利用所有可用于frugiperda的EST序列设计了基因表达芯片。然后将其用于比较抗性菌株与敏感参考菌株的基因表达。先前在其他昆虫中涉及代谢抗性的几个基因家族成员被发现在抗性菌株中过度表达，包括谷胱甘肽s转移酶、细胞色素p450和羧酸酯酶。综上所述，这些结果提供了证据，表明果giperda对拟除虫菊酯类杀虫剂和有机磷的抗性是基于靶点和代谢机制。
The fall armyworm Spodoptera frugiperda is an economically important pest of small grain crops that occurs in all maize growing regions of the Americas. The intensive use of chemical pesticides for its control has led to the selection of resistant populations, however, to date, the molecular mechanisms underlying resistance have not been characterised. In this study the mechanisms involved in the resistance of two S. frugiperda strains collected in Brazil to chlorpyrifos (OP strain) or lambda-cyhalothrin (PYR strain) were investigated using molecular and genomic approaches. To examine the possible role of target-site insensitivity the genes encoding the organophosphate (acetylcholinesterase, AChE) and pyrethroid (voltage-gated sodium channel, VGSC) target-site proteins were PCR amplified. Sequencing of the S. frugiperda ace-1 gene identified several nucleotide changes in the OP strain when compared to a susceptible reference strain (SUS). These result in three amino acid substitutions, A201S, G227A and F290V, that have all been shown previously to confer organophosphate resistance in several other insect species. Sequencing of the gene encoding the VGSC in the PYR strain, identified mutations that result in three amino acid substitutions, T929I, L932F and L1014F, all of which have been shown previously to confer knockdown/super knockdown-type resistance in several arthropod species. To investigate the possible role of metabolic detoxification in the resistant phenotype of the OP and PYR stains all EST sequences available for S. frugiperda were used to design a gene-expression microarray. This was then used to compare gene expression in the resistant strains with the susceptible reference strain. Members of several gene families, previously implicated in metabolic resistance in other insects were found to be overexpressed in the resistant strains including glutathione S-transferases, cytochrome P450s and carboxylesterases. Taken together these results provide evidence that both target-site and metabolic mechanisms underlie the resistance of S. frugiperda to pyrethroids and organophosphates.
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影响因子：
3.8
作者：
LAGADIC, L;CUANY, A;ECHAUBARD, M
通讯作者：
ECHAUBARD, M
影响因子：
2.2
作者：
Rios-Diez, J. D.;Saldamando-Benjumea, C. I.
通讯作者：
Saldamando-Benjumea, C. I.
影响因子：
4.1
作者：
Fontaine, Severine;Caddoux, Laetitia;Roy, Lise
通讯作者：
Roy, Lise
影响因子：
3.8
作者：
Kostaropoulos, I;Papadopoulos, AI;Papadopoulou-Mourkidou, E
通讯作者：
Papadopoulou-Mourkidou, E
影响因子：
3
作者：
Nègre V;Hôtelier T;Volkoff AN;Gimenez S;Cousserans F;Mita K;Sabau X;Rocher J;López-Ferber M;d'Alençon E;Audant P;Sabourault C;Bidegainberry V;Hilliou F;Fournier P
通讯作者：
Fournier P