SPODOBASE: an EST database for the lepidopteran crop pest Spodoptera.
SPODOBASE: an EST database for the lepidopteran crop pest Spodoptera.
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DOI：
10.1186/1471-2105-7-322
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发表时间：
2006-06-23
影响因子：
3
通讯作者：
Fournier P
中科院分区：
文献类型：
--
作者：
Nègre V;Hôtelier T;Volkoff AN;Gimenez S;Cousserans F;Mita K;Sabau X;Rocher J;López-Ferber M;d'Alençon E;Audant P;Sabourault C;Bidegainberry V;Hilliou F;Fournier P
鳞翅目夜蛾是一种对多种农作物造成广泛经济损失的害虫。它还因其著名的Sf9细胞系而闻名，该细胞系用于大量异源蛋白质的生产。夜蛾属的物种被用作研究杀虫剂抗药性和研究病毒宿主相互作用的模型。基因组方法现在是这些昆虫生物学和病理学进一步发展的关键一步，EST测序工作的结果需要被组织到数据库中，提供一套综合的工具和信息。从三种不同的果蝇组织(血细胞、中肠和脂肪体)和Sf9细胞系制备的五个独立的cDNA文库中的EST被保存在数据库中。之所以选择这些组织，是因为它们在免疫反应、发育和植物/昆虫相互作用等生物过程中具有重要作用。到目前为止，SPODOBASE包含29,325个EST，它们被清理并聚类到非冗余集合(2294个集群和6103个单体)。SPODOBASE的构建方式是，可以添加来自果蝇或其他物种的其他EST。用户可以使用文本搜索、预格式化查询、查询助手或BLAST搜索来检索信息。针对NCBI、UniProt或Bombyx mori Est数据库以及Go-Slim词汇表提供注释。SPODOBASE数据库提供了对鳞翅目昆虫果翅夜蛾表达序列标签(EST)的集成访问。这是一个公开可用的结构化昆虫序列数据库，将允许识别一些基因并全面克隆科学界感兴趣的基因家族。可从以下URL获得SPODOBASE：
The Lepidoptera Spodoptera frugiperda is a pest which causes widespread economic damage on a variety of crop plants. It is also well known through its famous Sf9 cell line which is used for numerous heterologous protein productions. Species of the Spodoptera genus are used as model for pesticide resistance and to study virus host interactions. A genomic approach is now a critical step for further new developments in biology and pathology of these insects, and the results of ESTs sequencing efforts need to be structured into databases providing an integrated set of tools and informations. The ESTs from five independent cDNA libraries, prepared from three different S. frugiperda tissues (hemocytes, midgut and fat body) and from the Sf9 cell line, are deposited in the database. These tissues were chosen because of their importance in biological processes such as immune response, development and plant/insect interaction. So far, the SPODOBASE contains 29,325 ESTs, which are cleaned and clustered into non-redundant sets (2294 clusters and 6103 singletons). The SPODOBASE is constructed in such a way that other ESTs from S. frugiperda or other species may be added. User can retrieve information using text searches, pre-formatted queries, query assistant or blast searches. Annotation is provided against NCBI, UNIPROT or Bombyx mori ESTs databases, and with GO-Slim vocabulary. The SPODOBASE database provides integrated access to expressed sequence tags (EST) from the lepidopteran insect Spodoptera frugiperda. It is a publicly available structured database with insect pest sequences which will allow identification of a number of genes and comprehensive cloning of gene families of interest for scientific community. SPODOBASE is available from URL:
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影响因子：
1.4
作者：
Molina-Ochoa, J;Lezama-Gutierrez, R;Arceo-Palacios, F
通讯作者：
Arceo-Palacios, F
影响因子：
3.8
作者：
Iglesias, F;Marco, P;Jacquin-Joly, E
通讯作者：
Jacquin-Joly, E
影响因子：
5.8
作者：
Landais, I;Ogliastro, M;Devauchelle, G
通讯作者：
Devauchelle, G
影响因子：
14.9
作者：
Dicks, J;Anderson, M;Waugh, R
通讯作者：
Waugh, R
影响因子：
5.8
作者：
Pertea, G;Huang, XQ;Quackenbush, J
通讯作者：
Quackenbush, J