Using synchrotron X-ray phase-contrast micro-computed tomography to study tissue damage by laser irradiation.
Using synchrotron X-ray phase-contrast micro-computed tomography to study tissue damage by laser irradiation.
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DOI：
10.1002/lsm.22571
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发表时间：
2016-11
影响因子：
2.4
通讯作者：
Richter CP
中科院分区：
文献类型：
--
作者：
Robinson AM;Stock SR;Soriano C;Xiao X;Richter CP
本研究的目的是确定x射线微计算机断层扫描是否可以用于定位和表征激光照射引起的组织损伤，并描述其在此应用中相对于传统组织学的优势。在不同功率设置下，以单脉冲模式（100毫秒）操作的外科CO2激光器用于烧蚀不同类型的尸体动物组织。然后收集组织样本，并使用同步加速器x射线相衬和微型计算机断层扫描成像，以生成组织的虚拟切片堆栈。随后，使用Fiji （ImageJ）软件定位组织损伤，然后从组织图像堆栈的3D渲染图中量化激光消融锥和热凝损伤的体积。将x射线图像中的组织结构与经典光学显微镜下可见的组织结构进行了视觉比较。我们证明了微型计算机断层扫描可以用于快速识别手术激光消融、空泡化、碳化和热凝固组织的区域。与传统的组织学方法相比，我们可以更快地定量和比较代表消融组织体积的消融坑和热凝区体积。我们证明了这些程序可以在新鲜的水合和未切片的塑料嵌入组织上进行。我们证明了应用非破坏性显微计算机断层扫描来可视化和分析激光诱导的组织损伤而不需要组织切片是可能的。这将提高对新型手术激光及其对组织的相应影响的评估。
The aim of this study was to determine if X-ray micro-computed tomography could be used to locate and characterize tissue damage caused by laser irradiation and to describe its advantages over classical histology for this application. A surgical CO2 laser, operated in single pulse mode (100 milliseconds) at different power settings, was used to ablate different types of cadaveric animal tissues. Tissue samples were then harvested and imaged with synchrotron X-ray phase-contrast and micro-computed tomography to generate stacks of virtual sections of the tissues. Subsequently, Fiji (ImageJ) software was used to locate tissue damage, then to quantify volumes of laser ablation cones and thermal coagulation damage from 3D renderings of tissue image stacks. Visual comparisons of tissue structures in X-ray images with those visible by classic light microscopy histology were made. We demonstrated that micro-computed tomography could be used to rapidly identify areas of surgical laser ablation, vacuolization, carbonization, and thermally coagulated tissue. Quantification and comparison of the ablation crater, which represents the volume of ablated tissue, and the thermal coagulation zone volumes were performed faster than we could by classical histology. We demonstrated that these procedures can be performed on fresh hydrated and non-sectioned plastic embedded tissue. We demonstrated that the application of non-destructive micro-computed tomography to the visualization and analysis of laser induced tissue damage without tissue sectioning is possible. This will improve evaluation of new surgical lasers and their corresponding effect on tissues.
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影响因子：
2.4
作者：
Taudorf, E. H.;Lerche, C. M.;Haedersdal, M.
通讯作者：
Haedersdal, M.
影响因子：
2.4
作者：
Estvold, Soren K.;Mordini, Frederico;Horvath, Keith A.
通讯作者：
Horvath, Keith A.
DOI：
10.1007/978-90-481-8831-4_13
发表时间：
2011-01-01
期刊：
OPTICAL THERMAL RESPONSE OF LASER-IRRADIATED TISSUE, SECOND EDITION
影响因子：
--
作者：
Thomsen, Sharon;Pearce, John A.
通讯作者：
Pearce, John A.
影响因子：
2
作者：
Paganin, D;Mayo, SC;Wilkins, SW
通讯作者：
Wilkins, SW
影响因子：
2.5
作者：
Gerneke, Dane A.;Sands, Gregory B.;Legrice, Ian J.
通讯作者：
Legrice, Ian J.