Antioxidants Attenuate Oxidative Stress-Induced Hidden Blood Loss in Rats.
Antioxidants Attenuate Oxidative Stress-Induced Hidden Blood Loss in Rats.
复制标题
抗氧化剂会减弱大鼠氧化应激诱导的隐藏失血。
DOI：
10.4274/tjh.2016.0469
复制
发表时间：
2017-12-01
期刊：
影响因子：
--
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Qian H;Yuan T;Tong J;Sun WS;Jin J;Chen WX;Meng J;Bao N;Zhao J
隐性失血(HBL)是全膝关节或髋关节置换术后常见的并发症，根据可见失血量，即使在回输或输血后也会导致术后贫血。最近的研究表明，氧化应激可能参与了HBL的发生。然而，抗氧化剂原花青素(PA)或氢水(HW)是否能改善HBL仍然知之甚少。本研究的目的是评价PA和HW对HBL的影响。用亚油酸加或不加PA或HW处理建立大鼠HBL模型。检测血红蛋白(Hb)、红细胞计数(RBC)、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、丙二醛(MDA)和血红蛋白铁基含量。给予亚油酸后，RBC、Hb值降低，SOD、GSHPx活性降低，PA或HW可使其改善。此外，给予PA或HW后，丙二醛含量显著降低。PA和HW可通过减轻氧化应激来改善大鼠模型的HBL，提示它们可作为临床上预防或治疗HBL的一种新的治疗方法。
Hidden blood loss (HBL), commonly seen after total knee or hip arthroplasty, causes postoperative anemia even after reinfusion or blood transfusion based on the visible blood loss volume. Recent studies demonstrated that oxidative stress might be involved in HBL. However, whether the antioxidants proanthocyanidin (PA) or hydrogen water (HW) can ameliorate HBL remains poorly understood. The aim of this study was to evaluate the effects of PA and HW on HBL. A rat HBL model was established through administration of linoleic acid with or without treatment with PA or HW. The levels of hemoglobin (Hb), red blood cell (RBC) count, superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-PX) activity, malondialdehyde (MDA), and ferryl Hb were measured. RBC and Hb values as well as the activity of SOD and GSHPX were reduced after administration of linoleic acid, which was ameliorated by treatment with PA or HW. In addition, the quantity of MDA was significantly decreased with the administration of PA or HW. PA and HW could ameliorate HBL in a rat model by reducing oxidative stress, suggesting that they might be used as a novel therapeutic approach in the prophylaxis or treatment of HBL in clinics.
登录
查看更多内容
影响因子：
6.1
作者：
Shen Hui-liang;Li Zheng;Cao Guang-le
通讯作者：
Cao Guang-le
影响因子：
8.8
作者：
Kato, N;Nakanishi, K;Ogawa, R
通讯作者：
Ogawa, R
DOI：
10.1016/j.pathophys.2012.05.001
发表时间：
2012-04-01
期刊：
Pathophysiology : the official journal of the International Society for Pathophysiology
影响因子：
--
作者：
Roy, Anandita;Sil, Parames C
通讯作者：
Sil, Parames C
影响因子：
2.6
作者：
Esmaeilnejad, Bijan;Tavassoli, Mosa;Dalir-Naghadeh, Bahram
通讯作者：
Dalir-Naghadeh, Bahram
影响因子：
2.6
作者：
Yoritaka A;Abe T;Ohtsuka C;Maeda T;Hirayama M;Watanabe H;Saiki H;Oyama G;Fukae J;Shimo Y;Hatano T;Kawajiri S;Okuma Y;Machida Y;Miwa H;Suzuki C;Kazama A;Tomiyama M;Kihara T;Hirasawa M;Shimura H;Hattori N
通讯作者：
Hattori N