Endophytic N2 fixation in sweet potato: responses to N, P, and K inputs and visualization of 15N2 utilizing bacterial cells via Raman spectroscopy
Endophytic N2 fixation in sweet potato: responses to N, P, and K inputs and visualization of 15N2 utilizing bacterial cells via Raman spectroscopy
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甘薯内生 N2 固定：对 N、P 和 K 输入的响应以及通过拉曼光谱利用细菌细胞可视化 15N2
DOI：
10.1007/s00374-023-01698-5
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发表时间：
2023
影响因子：
6.5
通讯作者：
Risako Ueda & Katsuya Yano
中科院分区：
文献类型：
--
作者：
鈴木颯人;岩田拓記;宮原裕一;伊藤雅之，高橋けんし;Risako Ueda & Katsuya Yano
氮肥强烈限制非豆科作物的产量；然而，甘薯（Ipomoea batatas）是一个例外，可能是因为它能够通过内生固氮生物获取大气中的氮气。利用拉曼光谱，我们发现在15N2喂养的甘薯中，一些内生细菌含有15N，为植物中N2固定提供了直接证据。为了评估固氮能力，通过改变氮、磷和钾肥投入进行了盆栽试验。甘薯的氮含量高于非固氮蕹菜，并且氮含量增加，比肥料氮输入高1.4倍。其 δ15N 值更接近固氮大豆，估计植物氮的 11-56% 来自 N2。在没有施氮肥的情况下，甘薯中固定氮的估计量可以忽略不计；然而，随着肥料氮的增加，观察到逐渐积累导致立即饱和。在此状态下，增加磷供应量线性增强了能力，达到 13 g N m−2，与豆类相当。然而，钾的输入既不影响固氮，也不影响生长，因为即使没有肥料钾，由于从土壤中吸收大量的钾。我们的结果表明，甘薯具有广泛的固氮作用，具体取决于其营养状况，特别是磷；共振拉曼光谱有助于在单细胞规模上观察活性固氮细菌。
Fertilizer-N strongly limits non-leguminous crop yields; however, sweet potato (Ipomoea batatas) is an exception, likely due to its ability to acquire atmospheric N2via endophytic diazotrophs. Using Raman spectroscopy, we found that in15N2-fed sweet potato, some endophytic bacteria contained15N, providing direct evidence of N2fixationin planta. To assess N2-fixing capability, pot experiments were conducted by varying N, P, and K fertilizer inputs. Sweet potato showed higher N content than the non-N2-fixingIpomoea aquatica;additionally, it showed increased N content which was 1.4-fold higher than the fertilizer-N input. Its δ15N values were closer to those of N2-fixing soybean, with an estimated 11–56% of plant N derived from N2. The estimated amount of fixed-N in sweet potato was negligible without fertilizer-N; however, a gradual accumulation leading to an immediate saturation was observed with increasing fertilizer-N. During this state, increasing P supply linearly enhanced the capability, reaching 13 g N m−2, comparable to that of legumes. However, K inputs affected neither N2fixation nor growth owing to strong K acquisition from the soil even without fertilizer-K. Our results indicate extensive N2fixation in sweet potato, depending on its nutritional status, particularly P; resonance Raman spectroscopy facilitates the visualization of active N2-fixing bacteria on a single-cell scale.
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甘薯对钾肥和磷肥的反应1
影响因子：
2.1
作者：
J. J. Nicholaides;H. Chancy;H. Mascagni;L. G. Wilson;D. W. Eaddy
通讯作者：
D. W. Eaddy
影响因子：
2.2
作者：
Terakado-Tonooka, Junko;Ohwaki, Yoshinari;Fujihara, Shinsuke
通讯作者：
Fujihara, Shinsuke
影响因子：
3.5
作者：
Daimon, H;Yoshioka, M
通讯作者：
Yoshioka, M
甘薯、南瓜、高粱和蓖麻子中 15N 的自然丰度：甘薯中 N2 源氮的可能输入
影响因子：
6.5
作者：
T. Yoneyama;J. Terakado;T. Masuda
通讯作者：
T. Masuda
影响因子：
2
作者：
Adachi, K;Nakatani, M;Mochida, H
通讯作者：
Mochida, H