Ancient T-independence of mucosal IgX/A: gut microbiota unaffected by larval thymectomy in Xenopus laevis.
Ancient T-independence of mucosal IgX/A: gut microbiota unaffected by larval thymectomy in Xenopus laevis.
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作者：
许多研究涉及肠道微生物群对免疫系统的影响，但很少有研究剖析T细胞对肠道微生物区系和粘膜反应的影响。我们采用非洲爪哇幼体胸腺切除的方法来研究T淋巴细胞对肠道微生物区系的影响。16S rRNA基因的焦磷酸测序用于评估胃、小肠和大肠中细菌群的相对丰度。肠杆菌科种类最多，其次为拟杆菌科、肠杆菌科和黄杆菌科。Unifrac分析表明，胸腺切除青蛙和未手术青蛙之间的微生物区系分布没有差异。这与免疫数据一致，免疫数据显示，胸腺切除对粘膜免疫球蛋白IgX水平没有显著影响。这项在非洲爪哇进行的研究代表了最古老的生物，表现出向粘膜同型的类别转换，并与哺乳动物免疫学有关，因为根据系统发育、基因组同步性和功能，IgA似乎是从IgX进化而来的。
Many studies address the influence of the gut microbiome on the immune system, but few dissect the effect of T cells on gut microbiota and mucosal responses. We have employed larval thymectomy in Xenopus to study the gut microbiota with and without the influence of T lymphocytes. Pyrosequencing of 16S rRNA genes was used to assess the relative abundance of bacterial groups present in the stomach, small and large intestine. Clostridiaceae was the most abundant family throughout the gut, while Bacteroidaceae, Enterobacteriaceae and Flavobacteriaceae also were well represented. Unifrac analysis revealed no differences in microbiota distribution between thymectomized and unoperated frogs. This is consistent with immunization data showing that levels of the mucosal immunoglobulin IgX are not altered significantly by thymectomy. This study in Xenopus represents the oldest organisms that exhibit class switch to a mucosal isotype and is relevant to mammalian immunology, as IgA appears to have evolved from IgX based upon phylogeny, genomic synteny, and function.
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DOI：
10.1084/jem.173.2.471
发表时间：
1991-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Guy-Grand D;Cerf-Bensussan N;Malissen B;Malassis-Seris M;Briottet C;Vassalli P
通讯作者：
Vassalli P
影响因子：
1
作者：
Choi, Song Yi;Suh, Kwang Sun;Lim, Beom Jin
通讯作者：
Lim, Beom Jin
影响因子：
2.9
作者：
Chida, Asiya Seema;Goyos, Ana;Robert, Jacques
通讯作者：
Robert, Jacques
影响因子：
5.4
作者：
DunnWalters, DK;Boursier, L;Spencer, J
通讯作者：
Spencer, J
影响因子：
64.8
作者：
Fritz, Joerg H.;Rojas, Olga Lucia;Simard, Nathalie;McCarthy, Douglas D.;Hapfelmeier, Siegfried;Rubino, Stephen;Robertson, Susan J.;Larijani, Mani;Gosselin, Jean;Ivanov, Ivaylo I.;Martin, Alberto;Casellas, Rafael;Philpott, Dana J.;Girardin, Stephen E.;McCoy, Kathy D.;Macpherson, Andrew J.;Paige, Christopher J.;Gommerman, Jennifer L.
通讯作者：
Gommerman, Jennifer L.