Two gut intraepithelial CD8+ lymphocyte populations with different T cell receptors: a role for the gut epithelium in T cell differentiation.
Two gut intraepithelial CD8+ lymphocyte populations with different T cell receptors: a role for the gut epithelium in T cell differentiation.
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DOI：
10.1084/jem.173.2.471
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发表时间：
1991-02-01
期刊：
影响因子：
--
通讯作者：
Vassalli P
中科院分区：
文献类型：
--
作者：
Guy-Grand D;Cerf-Bensussan N;Malissen B;Malassis-Seris M;Briottet C;Vassalli P
小鼠肠道上皮内淋巴细胞(IEL)主要由两类CD8+T细胞组成(90%)。其中一个含有异二聚体的α/βCD8链(Lyt-2+，Lyt-3+)，这是一种由α/β链组成的T细胞受体(TCR)，它是Thy-1+；它代表由抗原刺激在Peyer氏斑中诱导的T母细胞的后代。另一种含有同源二聚体的α/αCD8+链，不含β链mRNA，主要是Thy-1-和TCR-Gamma/Delta+或-α/beta+，其存在表明它不依赖胸腺，不需要抗原刺激，其存在表明：(A)在裸鼠和SCID小鼠中；(B)在照射和切除胸腺的小鼠中，由带有可识别标记的T耗竭的骨髓细胞重新填充；(C)在胸腺切除的小鼠中，注射抗CD8的单抗，导致外周CD8+T淋巴细胞完全耗尽；以及(D)在无菌小鼠和哺乳小鼠中。在幼年裸鼠的IEL上发现了α/αCD8链、CD3-TCR复合体和TCR mRNAs(第一伽马/增量)，而在外周或循环中的淋巴细胞或骨髓细胞上或在其中检测不到它们。与成熟的T细胞不同，IEL含有RAG蛋白的mRNA，这是TCR和Ig基因重排所必需的。我们认为，肠道上皮(一种内胚层的衍生物，如胸腺上皮)具有诱导性，可以吸引骨髓来源的祖细胞，并触发它们的TCR重排和α/αCD8链的表达，从而产生一个似乎与γ/β胸腺细胞属于同一谱系的T细胞群体，并识别不同于α/βCD8+IEL的抗原谱。
Mouse gut intraepithelial lymphocytes (IEL) consist mainly (90%) of two populations of CD8+ T cells. One bears heterodimeric alpha/beta CD8 chains (Lyt-2+, Lyt-3+), a T cell receptor (TCR) made of alpha/beta chains, and is Thy-1+; it represents the progeny of T blasts elicited in Peyer's patches by antigenic stimulation. The other bears homodimeric alpha/alpha CD8+ chains, contains no beta chain mRNA, and is mostly Thy-1- and TCR-gamma/delta + or -alpha/beta +; it is thymo- independent and does not require antigenic stimulation, as shown by its presence: (a) in nude and scid mice; (b) in irradiated and thymectomized mice repopulated by T-depleted bone marrow cells bearing an identifiable marker; (c) in thymectomized mice treated by injections of monoclonal anti-CD8 antibody, which lead to total depletion of peripheral CD8+ T lymphocytes; and (d) in germ-free mice and in suckling mice. In young nude mice, alpha/alpha CD8 chains, CD3-TCR complexes, and TCR mRNAs (first gamma/delta) are found on IEL, while they are not detectable on or in peripheral or circulating lymphocytes or bone marrow cells. IEL, in contrast to mature T cells, contain mRNA for the RAG protein, which is required for the rearrangement of TCR and Ig genes. We propose that the gut epithelium (an endoderm derivative, as the thymic epithelium) has an inductive property, attracting progenitors of bone marrow origin, and triggering their TCR rearrangement and alpha/alpha CD8 chains expression, thus giving rise to a T cell population that appears to belong to the same lineage as gamma/delta thymocytes and to recognize an antigenic repertoire different from that of alpha/beta CD8+ IEL.
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DOI：
10.1084/jem.160.1.12
发表时间：
1984-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Guy-Grand D;Dy M;Luffau G;Vassalli P
通讯作者：
Vassalli P
影响因子：
15.9
作者：
GUYGRAND, D;VASSALLI, P
通讯作者：
VASSALLI, P
影响因子：
5.4
作者：
CERFBENSUSSAN, N;JARRY, A;GRISCELLI, C
通讯作者：
GRISCELLI, C
影响因子：
64.8
作者：
CHIEN, YH;IWASHIMA, M;DAVIS, MM
通讯作者：
DAVIS, MM
影响因子：
64.8
作者：
BONNEVILLE, M;JANEWAY, CA;TONEGAWA, S
通讯作者：
TONEGAWA, S