Expression profiling of potato germplasm differentiated in quality traits leads to the identification of candidate flavour and texture genes.
Expression profiling of potato germplasm differentiated in quality traits leads to the identification of candidate flavour and texture genes.
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DOI：
10.1093/jxb/ern264
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发表时间：
2008
影响因子：
6.9
通讯作者：
Taylor MA
中科院分区：
文献类型：
--
作者：
Ducreux LJ;Morris WL;Prosser IM;Morris JA;Beale MH;Wright F;Shepherd T;Bryan GJ;Hedley PE;Taylor MA
品质性状，如风味和质地，在作物育种计划中越来越重要。本研究利用马铃薯种质在块茎风味和质构性状上的分化。最近开发的44000元素马铃薯微阵列用于鉴定块茎基因表达谱，这些基因表达谱对应于块茎风味和质地的差异以及类胡萝卜素含量和休眠特征。比较了两个龙葵组（Phureja）和两个龙葵组（tuberosum）的基因表达；在Phureja中，309个基因持续显著上调，555个基因下调。这些列表中大约46%的基因可以从它们的注释中识别出来，其中一些候选基因可能支持Phureja/Tuberosum性状差异。例如，煮熟的块茎挥发性谱的明显差异是，普瑞亚的倍半萜α-copaene含量高于Tuberosum。在普柳块茎中发现了一个高表达倍半萜合成酶基因，并证实其全长cDNA编码α-copaene合成酶。其他潜在的“风味基因”，从它们的差异表达谱中鉴定出来，包括编码支链氨基酸转氨酶和核糖核酸酶的基因，这表明土豆块茎在烹饪时形成5 ' -核糖核苷酸的机制。还鉴定了参与细胞壁生物合成（以及潜在质地）的基因表达水平的主要差异，包括编码果胶乙酰酯酶、木葡聚糖内转糖基化酶和果胶甲基酯酶的基因。其他可能影响块茎类胡萝卜素含量和块茎生命周期表型的基因表达差异也进行了讨论。
Quality traits such as flavour and texture are assuming a greater importance in crop breeding programmes. This study takes advantage of potato germplasm differentiated in tuber flavour and texture traits. A recently developed 44 000-element potato microarray was used to identify tuber gene expression profiles that correspond to differences in tuber flavour and texture as well as carotenoid content and dormancy characteristics. Gene expression was compared in two Solanum tuberosum group Phureja cultivars and two S. tuberosum group Tuberosum cultivars; 309 genes were significantly and consistently up-regulated in Phureja, whereas 555 genes were down-regulated. Approximately 46% of the genes in these lists can be identified from their annotation and amongst these are candidates that may underpin the Phureja/Tuberosum trait differences. For example, a clear difference in the cooked tuber volatile profile is the higher level of the sesquiterpene α-copaene in Phureja compared with Tuberosum. A sesquiterpene synthase gene was identified as being more highly expressed in Phureja tubers and its corresponding full-length cDNA was demonstrated to encode α-copaene synthase. Other potential ‘flavour genes’, identified from their differential expression profiles, include those encoding branched-chain amino acid aminotransferase and a ribonuclease suggesting a mechanism for 5′-ribonucleotide formation in potato tubers on cooking. Major differences in the expression levels of genes involved in cell wall biosynthesis (and potentially texture) were also identified, including genes encoding pectin acetylesterase, xyloglucan endotransglycosylase and pectin methylesterase. Other gene expression differences that may impact tuber carotenoid content and tuber life-cycle phenotypes are discussed.
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影响因子：
3.1
作者：
Aubourg, S;Lecharny, A;Bohlmann, J
通讯作者：
Bohlmann, J
影响因子：
7.4
作者：
Fernie, AR;Willmitzer, L
通讯作者：
Willmitzer, L
影响因子：
2.1
作者：
Beck, HC
通讯作者：
Beck, HC
影响因子：
56.9
作者：
Johanson, U;West, J;Dean, C
通讯作者：
Dean, C
影响因子：
5.4
作者：
Ghislain, M.;Andrade, D.;Spooner, D. M.
通讯作者：
Spooner, D. M.