Sphingoid bases are taken up by Escherichia coli and Staphylococcus aureus and induce ultrastructural damage.
Sphingoid bases are taken up by Escherichia coli and Staphylococcus aureus and induce ultrastructural damage.
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DOI：
10.1159/000343175
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发表时间：
2013
影响因子：
2.7
通讯作者：
Wertz PW
中科院分区：
文献类型：
--
作者：
Fischer CL;Walters KS;Drake DR;Blanchette DR;Dawson DV;Brogden KA;Wertz PW
在皮肤外层发现的狮身人面像碱基对革兰氏阳性和革兰氏阴性细菌具有抗菌活性。我们研究了大肠杆菌和金黄色葡萄球菌对几种狮身人面像碱基的摄取，并评估了随后的超微结构损伤。将D-鞘氨醇、二氢鞘氨醇或植酸鞘氨醇分别与大肠杆菌和金黄色葡萄球菌以10倍的最低抑菌浓度孵育0.5h和4h，杀灭率分别为50%。处理后的细菌细胞立即制备扫描电子显微镜、透射电子显微镜，并用QTLC分析脂类含量。经狮身人掌碱基处理的大肠杆菌和金黄色葡萄球菌表面变形，表面凹凸不平。在使用二氢鞘氨醇和鞘氨醇(p<；0.0001)而不是植鞘氨醇时，大肠杆菌和金黄色葡萄球菌的视觉表面积与对照相比有显著差异。狮身人面像碱处理的金黄色葡萄球菌表现出细胞壁和细胞膜的破坏和丢失，而大肠杆菌的细胞膜看起来完好无损，外膜没有受到损害。大肠杆菌和金黄色葡萄球菌细胞都含有独特的内部包涵体，可能与细胞死亡有关。QTLC显示细菌广泛摄取狮身人面像碱基。因此，狮身人面像碱基引起细胞外和细胞内的损伤，并引起细胞内的包裹体，这可能反映了脂质的摄取。
Sphingoid bases found in the outer layers of the skin exhibit antimicrobial activity against Gram-positive and Gram-negative bacteria. We investigated the uptake of several sphingoid bases by Escherichia coli and Staphylococcus aureus, and assessed subsequent ultrastructural damage. E. coli and S. aureus were incubated with D-sphingosine, dihydrosphingosine, or phytosphingosine at ten times their MIC for 0.5 h and 4 h, respectively, to kill 50% of viable bacteria. Treated bacterial cells were immediately prepared for SEM, TEM, and analyzed for lipid content by QTLC. E. coli and S. aureus treated with sphingoid bases were distorted and their surfaces were concave and rugate. Significant differences were observed in the visual surface area relative to controls for both E. coli and S. aureus when treated with dihydrosphingosine and sphingosine (p<0.0001) but not phytosphingosine. While sphingoid base-treated S. aureus exhibited disruption and loss of cell wall and membrane, E. coli cytoplasmic membranes appeared intact and the outer envelope uncompromised. Both E. coli and S. aureus cells contained unique internal inclusion bodies, likely associated with cell death. QTLC demonstrated extensive uptake of sphingoid bases by the bacteria. Hence, sphingoid bases induce both extracellular and intracellular damage and cause intracellular inclusions that may reflect lipid uptake.
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影响因子：
11.8
作者：
Klein E;Smith DL;Laxminarayan R
通讯作者：
Laxminarayan R
影响因子：
2.8
作者：
Bergsson, G;Arnfinnsson, J;Thormar, H
通讯作者：
Thormar, H
DOI：
10.1073/pnas.93.1.412
发表时间：
1996-01-09
影响因子：
11.1
作者：
Brogden, KA;DeLucca, AJ;Elliott, S
通讯作者：
Elliott, S
影响因子：
4.8
作者：
Harder, J;Bartels, J;Schröder, JM
通讯作者：
Schröder, JM
影响因子：
6.5
作者：
Arikawa, J;Ishibashi, M;Imokawa, G
通讯作者：
Imokawa, G