AND-gate contrast agents for enhanced fluorescence-guided surgery.
AND-gate contrast agents for enhanced fluorescence-guided surgery.
复制标题
用于增强荧光引导手术的与门型造影剂。
DOI：
10.1038/s41551-020-00616-6
复制
发表时间：
2021-03
影响因子：
28.1
通讯作者：
Bogyo M
中科院分区：
文献类型：
--
作者：
Widen JC;Tholen M;Yim JJ;Antaris A;Casey KM;Rogalla S;Klaassen A;Sorger J;Bogyo M
肿瘤的手术切除需要精确确定病变与正常组织之间的位置及边界。然而，不规则的边界使得这一过程困难重重。尽管分子靶向光学造影剂可在手术中实时界定肿瘤边界，但由于靶点在健康组织和肿瘤组织中均有表达，造影剂的选择性往往受到限制。在此，我们展示了 “与” 门光学成像探针，该探针需多种肿瘤特异性酶对两种底物进行处理，从而产生对肿瘤组织具有显著提高的特异性和敏感性的荧光信号。我们在乳腺肿瘤和转移性肺癌小鼠模型以及荧光引导机器人手术中评估了这些探针的性能。依赖多变量激活以选择性靶向复杂酶活性模式的成像探针，应有助于疾病的检测、治疗和监测。
The surgical resection of tumours requires the precise location and definition of the margins between lesions and normal tissue. However, this is made difficult by irregular margin borders. Although molecularly targeted optical contrast agents can be used to define tumour margins during surgery in real time, the selectivity of the contrast agents is often limited by the target being expressed in both healthy and tumour tissues. Here, we show that AND-gate optical imaging probes requiring the processing of two substrates by multiple tumour-specific enzymes produce a fluorescent signal with significantly improved specificity and sensitivity to tumour tissue. We evaluated the performance of the probes in mouse models of mammary tumours and of metastatic lung cancer, and during fluorescence-guided robotic surgery. Imaging probes relying on multivariate activation to selectively target complex patterns of enzymatic activity should be useful in disease detection, treatment and monitoring.
登录
查看更多内容
荧光引导的手术和实时导航 - 一种新的尖端。
DOI：
10.1038/nrc3566
发表时间：
2013-09
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1002/prca.201300105
发表时间：
2014-06
期刊：
Proteomics. Clinical applications
影响因子：
--
作者：
Aggarwal N;Sloane BF
通讯作者：
Sloane BF
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
4.6
作者：
Orosco RK;Tapia VJ;Califano JA;Clary B;Cohen EEW;Kane C;Lippman SM;Messer K;Molinolo A;Murphy JD;Pang J;Sacco A;Tringale KR;Wallace A;Nguyen QT
通讯作者：
Nguyen QT
影响因子：
28.4
作者：
Morrow, Monica;Abrahamse, Paul;Jagsi, Reshma
通讯作者：
Jagsi, Reshma