Purification of the total steroidal saponins from fenugreek seeds (Trigonella foenum-graecum L.) using aqueous two-phase system and determination of diosgenin content using micellar electrokinetic chromatography method
Purification of the total steroidal saponins from fenugreek seeds (Trigonella foenum-graecum L.) using aqueous two-phase system and determination of diosgenin content using micellar electrokinetic chromatography method
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水两相体系纯化胡芦巴种子（Trigonella foenum-graecum L.）中的总甾体皂苷并用胶束电动色谱法测定薯蓣皂苷元含量
DOI：
10.1080/14786419.2018.1455047
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发表时间：
2019-02
影响因子：
2.2
通讯作者：
Li Hailing
中科院分区：
文献类型：
--
作者：
Wang Na;He Famei;Li Wenliang;Fang Xu;Li Hailing
总甾体皂苷，特别是主要甾体皂苷元（薯蓣皂苷元）是胡芦巴籽提取物的主要活性成分。本研究采用乙醇-盐双水相体系（ATPS）纯化总甾体皂苷，并利用响应面法（RSM）对工艺条件进行优化。在优化条件下，总甾体皂苷在ATPS顶相的RSM预测回收率为97.9%，与实验平均回收率（98.3±4.2% (n = 6)）基本一致。建立了快速测定薯蓣提取物中薯蓣皂苷元的胶束电动色谱法。ATPS顶相提取物的薯蓣皂苷元含量是粗提取物的3倍，表明该ATPS具有纯化葫芦巴种子药理活性成分的巨大潜力。
Abstract The total steroidal saponins, particularly its major steroidal sapogenin (diosgenin), are the main active principles of fenugreek seed extract. In this study, an ethanol-salt aqueous two-phase system (ATPS) was explored for the purification of the total steroidal saponins, and the process conditions were optimized by response surface methodology (RSM). Under the optimized conditions, the RSM predicted recovery of the total steroidal saponins in the top phase of ATPS was 97.9%, which agreed with the average experimental recovery (98.3 ± 4.2% (n = 6)). Moreover, a rapid micellar electrokinetic chromatography (MEKC) method was developed for the determination of diosgenin from extracts. The diosgenin content in the ATPS top phase extract was 3-fold higher than that in crude extract, suggesting this ATPS having a great potential for purification pharmacological active ingredients from fenugreek seeds.
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DOI：
10.1002/pca.954
发表时间：
2007
期刊：
Phytochemical analysis : PCA
影响因子：
--
作者：
B. Kaufmann;S. Rudaz;S. Cherkaoui;J. Veuthey;P. Christen
通讯作者：
B. Kaufmann;S. Rudaz;S. Cherkaoui;J. Veuthey;P. Christen
影响因子：
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Li, Yan-Hua
影响因子：
6.1
作者：
Wesley G. Taylor;James L. Elder;P. Chang;Ken W. Richards
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DOI：
10.1111/j.1365-2621.2012.03082.x
发表时间：
2012-10
影响因子：
3.3
作者：
Xin Zhang;Fei Xu;Yuan Gao;Jinghe Wu;Yi Sun;Xiaoxiong Zeng
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Xin Zhang;Fei Xu;Yuan Gao;Jinghe Wu;Yi Sun;Xiaoxiong Zeng