Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical Detection of Cancerous Exosomes.
Aptasensor with Expanded Nucleotide Using DNA Nanotetrahedra for Electrochemical Detection of Cancerous Exosomes.
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DOI：
10.1021/acsnano.7b00373
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发表时间：
2017-04-25
期刊：
影响因子：
17.1
通讯作者：
Tan W
中科院分区：
文献类型：
--
作者：
Wang S;Zhang L;Wan S;Cansiz S;Cui C;Liu Y;Cai R;Hong C;Teng IT;Shi M;Wu Y;Dong Y;Tan W
外泌体是在生物流体中循环的细胞外囊泡（50-100 nm），作为细胞间信号传递器。尽管癌性外泌体作为肿瘤生物标志物的潜力很有希望，但敏感且快速地检测外泌体仍然具有挑战性。在此，我们结合了先进的适体技术，基于DNA的纳米结构和便携式电化学装置的优势，开发了一种用于直接捕获和检测肝细胞外泌体的纳米四面体（NTH）辅助适体传感器。适体的定向固定显著提高了含人工核碱基的适体对悬浮的外泌体的可及性，并且与单链适体官能化的适体传感器相比，NTH辅助的适体传感器可以以100倍的灵敏度检测外泌体。本研究为肿瘤源性外泌体的灵敏和有效定量提供了概念验证。因此，我们期望NTH辅助的电化学适体传感器成为全面的外泌体研究的有力工具。
Exosomes are extracellular vesicles (50–100 nm) circulating in biofluids as intercellular signal transmitters. Although the potential of cancerous exosomes as tumor biomarkers is promising, sensitive and rapid detection of exosomes remains challenging. Herein, we combined the strengths of advanced aptamer technology, DNA-based nanostructure, and portable electrochemical device to develop a nanotetrahedron (NTH)-assisted aptasensor for direct capture and detection of hepatocellular exosomes. The oriented immobilization of aptamers significantly improved the accessibility of an artificial nucleobase-containing aptamer to suspended exosomes, and the NTH-assisted aptasensor could detect exosomes with 100-fold higher sensitivity when compared to the single-stranded aptamer-functionalized aptasensor. The present study provides a proof-of-concept for sensitive and efficient quantification of tumor-derived exosomes. We thus expect the NTH-assisted electrochemical aptasensor to become a powerful tool for comprehensive exosome studies.
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