Suppression of chorismate synthase, which is localized in chloroplasts and peroxisomes, results in abnormal flower development and anthocyanin reduction in petunia
Suppression of chorismate synthase, which is localized in chloroplasts and peroxisomes, results in abnormal flower development and anthocyanin reduction in petunia
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抑制位于叶绿体和过氧化物酶体中的分支酸合酶，导致矮牵牛花发育异常和花青素减少
DOI：
10.1038/s41598-020-67671-6
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发表时间：
2020-07
影响因子：
4.6
通讯作者：
Yixun Yu
中科院分区：
文献类型：
--
作者：
Shiwei Zhong;Zeyu Chen;Jinyi Han;Huina Zhao;Juanxu Liu;Yixun Yu
摘要植物体中的莽草酸合成途径是芳香族氨基酸合成的重要途径。分支酸合成酶（CS）催化5-烯醇式莽草酸3-磷酸（EPSP）转化为分支酸的最后一步，但CS在高等植物代谢中的作用尚未见报道。在本研究中，我们发现矮牵牛（Petunia hybrida）中由单拷贝基因编码的PhCS含有N端质体转运肽和过氧化物酶体靶向信号。绿色荧光蛋白（GFP）融合蛋白测定显示，PhCS位于叶绿体，出乎意料的是，在过氧化物酶体。通过病毒诱导的基因沉默产生具有降低的PhCS活性的矮牵牛植物并进一步表征。PhCS沉默导致CS活性降低、严重生长迟缓、花和叶发育异常以及叶酸和色素（包括叶绿素、类胡萝卜素和花青素）水平降低。一个广泛针对性的代谢组学分析表明，大多数初级和次级代谢产物显着改变pTRV 2-PhCS处理的花冠。总的来说，结果揭示了与矮牵牛中的莽草酸途径相关的初级和专门代谢之间的明确联系。
AbstractIn plants, the shikimate pathway generally occurs in plastids and leads to the biosynthesis of aromatic amino acids. Chorismate synthase (CS) catalyses the last step of the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to chorismate, but the role of CS in the metabolism of higher plants has not been reported. In this study, we found that PhCS, which is encoded by a single-copy gene in petunia (Petunia hybrida), contains N-terminal plastidic transit peptides and peroxisomal targeting signals. Green fluorescent protein (GFP) fusion protein assays revealed that PhCS was localized in chloroplasts and, unexpectedly, in peroxisomes. Petunia plants with reduced PhCS activity were generated through virus-induced gene silencing and further characterized. PhCS silencing resulted in reduced CS activity, severe growth retardation, abnormal flower and leaf development and reduced levels of folate and pigments, including chlorophylls, carotenoids and anthocyanins. A widely targeted metabolomics analysis showed that most primary and secondary metabolites were significantly changed in pTRV2-PhCS-treated corollas. Overall, the results revealed a clear connection between primary and specialized metabolism related to the shikimate pathway in petunia.
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影响因子：
4.3
作者：
D. Mousdale;J. Coggins
通讯作者：
D. Mousdale;J. Coggins
影响因子：
4.6
作者：
Yang W;Cai Y;Hu L;Wei Q;Chen G;Bai M;Wu H;Liu J;Yu Y
通讯作者：
Yu Y
分类信号，N末端修饰和叶绿体蛋白质组的丰度。
影响因子：
3.7
作者：
Zybailov, Boris;Rutschow, Heidi;Friso, Giulia;Rudella, Andrea;Emanuelsson, Olof;Sun, Qi;van Wijk, Klaas J.
通讯作者：
van Wijk, Klaas J.
DOI：
10.1016/s0021-9258(18)54657-3
发表时间：
1991-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Andreas Schaller;J. Schmid;U. Leibinger;N. Amrhein
通讯作者：
Andreas Schaller;J. Schmid;U. Leibinger;N. Amrhein
影响因子：
3.5
作者：
D. Mousdale;J. Coggins
通讯作者：
D. Mousdale;J. Coggins