Three-dimensional laser micro- and nano-structuring of acrylated poly(ethylene glycol) materials and evaluation of their cytoxicity for tissue engineering applications.
Three-dimensional laser micro- and nano-structuring of acrylated poly(ethylene glycol) materials and evaluation of their cytoxicity for tissue engineering applications.
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DOI：
10.1016/j.actbio.2010.10.023
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发表时间：
2011-03
影响因子：
9.7
通讯作者：
Haverich, A.
中科院分区：
文献类型：
--
作者：
Ovsianikov, A.;Malinauskas, M.;Schlie, S.;Chichkov, B.;Gittard, S.;Narayan, R.;Loebler, M.;Sternberg, K.;Schmitz, K. -P.;Haverich, A.
天然细胞环境的特征在于复杂的三维结构，其中包含多个长度尺度的特征。许多三维细胞行为的体外研究依赖于具有受控三维拓扑结构的人工支架的可用性。在本文中，我们展示了制造的三维支架的组织工程的聚（乙二醇）二丙烯酸酯（PEGda）材料通过双光子聚合（2PP）。这种激光纳米结构化方法为快速制造具有任意几何形状的三维结构提供了独特的可能性。两个PEGda配方，其特征在于分子量为302和742的空间分辨率依赖于所施加的辐照参数进行了研究。我们证明，在这两种材料中获得的最小特征尺寸为200 nm。此外，还对材料配方的细胞毒性（光引发剂类型和光引发剂浓度）进行了广泛研究。来自光聚合的PEGda样品的水提取物表明存在水溶性分子，其对成纤维细胞有毒。结果表明，样品在水介质中老化，降低了这些提取物的细胞毒性，这种机制提供了一种途径，为生物医学应用的结构产生的2PP微细加工和光聚合技术一般。最后，确定了PEGda和光引发剂的完全生物相容性组合。制造可重复的支架结构对于系统地研究三维细胞过程和更好地理解体外组织形成是非常重要的。这项工作的结果表明，2PP可用于将聚（乙二醇）基材料组装成三维结构，具有明确的几何形状，模拟天然细胞环境的物理和生物学特性。
The natural cell environment is characterised by complex three-dimensional structures, which contain features at multiple length scales. Many in vitro studies of cell behaviour in three dimensions rely on the availability of artificial scaffolds with controlled three-dimensional topologies. In this paper, we demonstrate fabrication of three-dimensional scaffolds for tissue engineering out of poly(ethylene glycol) diacrylate (PEGda) materials by means of two-photon polymerization (2PP). This laser nanostructuring approach offers unique possibilities for rapid manufacturing of three-dimensional structures with arbitrary geometries. The spatial resolution dependence on the applied irradiation parameters is investigated for two PEGda formulations, which are characterized by molecular weights of 302 and 742. We demonstrate that minimum feature sizes of 200 nm are obtained in both materials. In addition, an extensive study of the cytotoxicity of the material formulations with respect to photoinitiator type and photoinitiator concentration is undertaken. Aqueous extracts from photopolymerized PEGda samples indicate the presence of water-soluble molecules, which are toxic to fibroblasts. It is shown that sample aging in aqueous medium reduces the cytotoxicity of these extracts; this mechanism provides a route for biomedical applications of structures generated by 2PP microfabrication and photopolymerization technologies in general. Finally, a fully biocompatible combination of PEGda and a photoinitiator is identified. Fabrication of reproducible scaffold structures is very important for systematic investigation of cellular processes in three dimensions and for better understanding of in vitro tissue formation. The results of this work suggest that 2PP may be used to polymerize poly(ethylene glycol)-based materials into three-dimensional structures with well-defined geometries that mimic the physical and biological properties of native cell environments.
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影响因子：
14
作者：
Mann, BK;Gobin, AS;West, JL
通讯作者：
West, JL
影响因子：
3.4
作者：
Hoffmann, Joseph C.;West, Jennifer L.
通讯作者：
West, Jennifer L.
影响因子：
14
作者：
Lee, Soo-Hong;Moon, James J.;West, Jennifer L.
通讯作者：
West, Jennifer L.
影响因子：
3.9
作者：
Huang, NP;Vörös, J;Spencer, ND
通讯作者：
Spencer, ND
影响因子：
14
作者：
Cai, SS;Liu, YC;Prestwich, GD
通讯作者：
Prestwich, GD