Low levels of interleukin-4 and high levels of transforming growth factor beta in rheumatoid synovitis.
Low levels of interleukin-4 and high levels of transforming growth factor beta in rheumatoid synovitis.
复制标题
类风湿性滑膜炎中白介素 4 水平低，转化生长因子 β 水平高。
作者：
Pierre Miossec;Mercedes Naviliat;A. D. d’Angeac;Jacques Sany;J. Banchereau
由于白细胞介素-4（IL-4）对T细胞和B细胞都有激动作用，我们研究了这种淋巴因子是否参与了类风湿性滑膜炎，这种疾病的特征是强烈的T细胞浸润和B细胞刺激。风湿性关节炎滑液（RA SF）不含（低于15 pg/ml）或含量极低（低于25 pg/ml）的IL-4，通过灵敏和特异性的酶联免疫吸附试验测定。未受刺激的类风湿性关节炎滑膜不产生IL-4。发现RA SF抑制佛波酯肉豆蔻酸酯醋酸酯（PMA）依赖的正常外周血淋巴细胞（PBL）增殖。通过凝胶过滤分离出表观分子量为150 kd的抑制级分。抑制组分对PMA、PMA + IL-2、PMA + IL-4诱导的PBL增殖有明显的抑制作用。然而，该组分对PMA + IL-2 + IL-4诱导的PBL增殖的阻断效果较差。在这些RA SF中发现高水平的转化生长因子β（TGF β），并且抗TGF β抗体能够部分降低抑制活性。发现RA SF抑制植物血凝素诱导的PBL产生IL-4。这些数据表明，IL-4，类似于其他T细胞淋巴因子，不能在RA SF中检测到，RA SF含有抑制活性，部分相关的TGF β，它阻止有丝分裂原诱导的PBL增殖，至少部分通过抑制T细胞衍生的淋巴因子释放。
Since interleukin-4 (IL-4) displays agonistic effects on both T and B cells, we studied whether this lymphokine is involved in rheumatoid synovitis, a disease characterized by intense T cell infiltration and B cell stimulation. Rheumatoid arthritis synovial fluids (RA SF) contained no (less than 15 pg/ml) or very low amounts (less than 25 pg/ml) of IL-4, as measured by a sensitive and specific enzyme-linked immunosorbent assay. No IL-4 was produced by unstimulated rheumatoid synovial membrane. RA SF were found to inhibit phorbol myristate acetate (PMA)-dependent proliferation of normal peripheral blood lymphocytes (PBL). An inhibitory fraction with an apparent molecular weight of 150 kd was isolated by gel filtration. The inhibitory fraction strongly blocked the proliferation of PBL induced by PMA, PMA + IL-2, or PMA + IL-4. However, this fraction was less effective in blocking the proliferation of PBL induced by PMA + IL-2 + IL-4. High levels of transforming growth factor beta (TGF beta) were found in these RA SF, and an anti-TGF beta antibody was able to partially reduce the inhibitory activity. RA SF were found to inhibit phytohemagglutinin-induced IL-4 production by PBL. These data indicate that IL-4, similar to other T cell lymphokines, cannot be detected in RA SF and that RA SF contains an inhibitory activity, related in part to TGF beta, which blocks mitogen-induced proliferation of PBL, at least in part through an inhibition of T cell-derived lymphokine release.
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影响因子：
2.2
作者：
LANDEGREN, U
通讯作者：
LANDEGREN, U
影响因子：
--
作者：
CUSH, JJ;LIPSKY, PE
通讯作者：
LIPSKY, PE
人转化生长因子 β.α 2-巨球蛋白复合物是转化生长因子 β 的潜在形式。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Huang,SS;O'Grady,P;Huang,JS
通讯作者：
Huang,JS
影响因子：
--
作者：
FIRESTEIN, GS;ZVAIFLER, NJ
通讯作者：
ZVAIFLER, NJ