DNA variation in the phenotypically-diverse brown alga Saccharina japonica.
DNA variation in the phenotypically-diverse brown alga Saccharina japonica.
复制标题
DOI：
10.1186/1471-2229-12-108
复制
发表时间：
2012-07-11
影响因子：
5.3
通讯作者：
Ayala FJ
中科院分区：
文献类型：
--
作者：
Balakirev ES;Krupnova TN;Ayala FJ
Saccharina japonica (Areschoug) Lane、Mayes、Druehl et Saunders 是一种经济上重要且形态高度可变的褐藻，栖息于西北太平洋海域。在核（ITS）、质体（rbcLS）和线粒体（COI）DNA序列数据的基础上，我们分析了典型的粳稻（TYP）及其两个常见形态变种（称为“长柄”（LON）和“浅水”（SHA）形式）的遗传组成，试图阐明它们的分类地位并评估粳稻中隐秘物种的可能性。数据显示，尽管在形态、生活史特征和生态偏好方面存在巨大差异，但 TYP 和 LON 形式在遗传上非常相似。然而，两者在基因上都与 SHA 形式截然不同。这两个糖精谱系通过 109 个固定单核苷酸差异以及 7 个固定长度多态性（基于包含三个基因区域的 4,286bp 连接数据集）来区分。 GenBank 数据库揭示了 TYP 和 LON 形式与 S. japonica 的密切亲和力，以及 SHA 形式与 S. cichorioides 的密切亲和力。本工作中使用的三个基因标记对于藻类鉴定具有不同的敏感性。 COI基因是最具辨别力的基因标记。然而，我们检测到了种间 COI 重组的实例，反映了远缘相关藻类谱系之间假定的历史杂交事件。重组序列在基因的 5'- 和 3'- 区域显示出高度对比的分歧水平，导致根据用于树重建的基因片段（5'-或 3'-）显着不同的树拓扑。因此，5'-COI“条形码”区域（约 650bp）可能会误导识别目的，至少对于可能经历过历史杂交事件的藻类物种而言是如此。考虑到表型可塑性在进化中的潜在作用，我们得出结论，TYP和LON形式代表了藻类表型多样化的例子，能够成功适应对比鲜明的浅水和深水海洋环境，而SHA形式与S. cichorioides非常相似，应被视为不同的物种。简要考虑了藻类管理和保护的实际应用。
Saccharina japonica (Areschoug) Lane, Mayes, Druehl et Saunders is an economically important and highly morphologically variable brown alga inhabiting the northwest Pacific marine waters. On the basis of nuclear (ITS), plastid (rbcLS) and mitochondrial (COI) DNA sequence data, we have analyzed the genetic composition of typical Saccharina japonica (TYP) and its two common morphological varieties, known as the “longipes” (LON) and “shallow-water” (SHA) forms seeking to clarify their taxonomical status and to evaluate the possibility of cryptic species within S. japonica. The data show that the TYP and LON forms are very similar genetically in spite of drastic differences in morphology, life history traits, and ecological preferences. Both, however, are genetically quite different from the SHA form. The two Saccharina lineages are distinguished by 109 fixed single nucleotide differences as well as by seven fixed length polymorphisms (based on a 4,286 bp concatenated dataset that includes three gene regions). The GenBank database reveals a close affinity of the TYP and LON forms to S. japonica and the SHA form to S. cichorioides. The three gene markers used in the present work have different sensitivity for the algal species identification. COI gene was the most discriminant gene marker. However, we have detected instances of interspecific COI recombination reflecting putative historical hybridization events between distantly related algal lineages. The recombinant sequences show highly contrasted level of divergence in the 5’- and 3’- regions of the gene, leading to significantly different tree topologies depending on the gene segment (5’- or 3’-) used for tree reconstruction. Consequently, the 5’-COI “barcoding” region (~ 650 bp) can be misleading for identification purposes, at least in the case of algal species that might have experienced historical hybridization events. Taking into account the potential roles of phenotypic plasticity in evolution, we conclude that the TYP and LON forms represent examples of algae phenotypic diversification that enables successful adaptation to contrasting shallow- and deep-water marine environments, while the SHA form is very similar to S. cichorioides and should be considered a different species. Practical applications for algal management and conservation are briefly considered.
登录
查看更多内容
影响因子：
3.3
作者：
Coyer, James A.;Hoarau, Galice;Olsen, Jeanine L.
通讯作者：
Olsen, Jeanine L.
横向遗传转移事件的系统发育鉴定。
影响因子：
3.4
作者：
Beiko RG;Hamilton N
通讯作者：
Hamilton N
影响因子：
4.9
作者：
Coyer, JA;Hoarau, G;Olsen, JL
通讯作者：
Olsen, JL
影响因子：
2.1
作者：
Coyer, J. A.;Hoarau, G.;Olsen, J. L.
通讯作者：
Olsen, J. L.
影响因子：
3.3
作者：
Ciborowski, Kate L.;Consuegra, Sofia;Jordan, William C.
通讯作者：
Jordan, William C.