Maternal Gut Microbiome Decelerates Fetal Endochondral Bone Formation by Inducing Inflammatory Reaction.
Maternal Gut Microbiome Decelerates Fetal Endochondral Bone Formation by Inducing Inflammatory Reaction.
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DOI：
10.3390/microorganisms10051000
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发表时间：
2022-05-10
期刊：
影响因子：
4.5
通讯作者：
Okamura, Hirohiko
中科院分区：
文献类型：
--
作者：
Uchida-Fukuhara, Yoko;Hattori, Takako;Fu, Shanqi;Kondo, Sei;Kuwahara, Miho;Fukuhara, Daiki;Islam, Md Monirul;Kataoka, Kota;Ekuni, Daisuke;Kubota, Satoshi;Morita, Manabu;Iikegame, Mika;Okamura, Hirohiko
为了探讨母体肠道微生物群对胎儿软骨内骨形成的影响，从无菌(GF)和无特定病原体(SPF)的孕妇那里获得了胚胎第18天的胎儿。胎儿全身的骨骼准备没有显示出明显的形态变化；然而，对胫骨的微型CT分析显示，SPF胫骨中的骨体积分数较低。原代培养的胎儿SPF肋骨软骨细胞表现出较低的细胞增殖能力和较低的细胞外基质积聚。RNA测序分析表明，在SPF软骨细胞中诱导了炎症相关基因，如白介素17受体、白介素6和免疫反应基因。这些数据表明，SPF小鼠的母体肠道微生物群影响胚胎软骨内成骨，可能是通过改变与炎症和免疫反应相关的基因在胎儿软骨中的表达。肠道微生物群可以改变穿过胎盘的胎儿软骨细胞的软骨内骨化。
To investigate the effect of the maternal gut microbiome on fetal endochondral bone formation, fetuses at embryonic day 18 were obtained from germ-free (GF) and specific-pathogen-free (SPF) pregnant mothers. Skeletal preparation of the fetuses’ whole bodies did not show significant morphological alterations; however, micro-CT analysis of the tibiae showed a lower bone volume fraction in the SPF tibia. Primary cultured chondrocytes from fetal SPF rib cages showed a lower cell proliferation and lower accumulation of the extracellular matrix. RNA-sequencing analysis showed the induction of inflammation-associated genes such as the interleukin (IL) 17 receptor, IL 6, and immune-response genes in SPF chondrocytes. These data indicate that the maternal gut microbiome in SPF mice affects fetal embryonic endochondral ossification, possibly by changing the expression of genes related to inflammation and the immune response in fetal cartilage. The gut microbiome may modify endochondral ossification in the fetal chondrocytes passing through the placenta.
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影响因子：
4.4
作者：
Erkkola, Maijaliisa;Nwaru, Bright I.;Virtanen, Suvi M.
通讯作者：
Virtanen, Suvi M.
DOI：
10.1016/j.bbi.2017.08.027
发表时间：
2018-01
期刊：
Brain, behavior, and immunity
影响因子：
--
作者：
Castillo-Ruiz A;Mosley M;George AJ;Mussaji LF;Fullerton EF;Ruszkowski EM;Jacobs AJ;Gewirtz AT;Chassaing B;Forger NG
通讯作者：
Forger NG
影响因子：
16.6
作者：
Lucas S;Omata Y;Hofmann J;Böttcher M;Iljazovic A;Sarter K;Albrecht O;Schulz O;Krishnacoumar B;Krönke G;Herrmann M;Mougiakakos D;Strowig T;Schett G;Zaiss MM
通讯作者：
Zaiss MM
影响因子：
15.5
作者：
Buford TW
通讯作者：
Buford TW
影响因子：
15.5
作者：
Berry ASF;Pierdon MK;Misic AM;Sullivan MC;O'Brien K;Chen Y;Murray SJ;Ramharack LA;Baldassano RN;Parsons TD;Beiting DP
通讯作者：
Beiting DP