How sticky? How tight? How hot? Imaging probes for fluid viscosity, membrane tension and temperature measurements at the cellular level.
How sticky? How tight? How hot? Imaging probes for fluid viscosity, membrane tension and temperature measurements at the cellular level.
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DOI：
10.1016/j.biocel.2022.106329
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发表时间：
2022-12
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我们回顾了三个重要的物理参数：粘度，膜张力和温度，所有这些都在许多细胞过程中发挥重要作用的成像探针的进展。最近的证据表明，细胞外液粘度可以调节细胞迁移速度;膜张力有助于调节细胞运动，外吞/内吞和细胞扩散面积;温度影响神经活动和脂肪细胞分化。我们讨论的技术实现基于成像的探针来测量粘度，膜张力和温度在亚细胞分辨率动态。每种技术的优点和缺点进行了检查，并探讨了最近开发的技术的未来应用。
We review the progress made in imaging probes for three important physical parameters: viscosity, membrane tension, and temperature, all of which play important roles in many cellular processes. Recent evidences showed that cell migration speed can be modulated by extracellular fluid viscosity; membrane tension contributes to the regulation of cell motility, exo-/endo-cytosis, and cell spread area; and temperature affects neural activity and adipocyte differentiation. We discuss the techniques implementing imaging-based probes to measure viscosity, membrane tension, and temperature at subcellular resolution dynamically. The merits and shortcomings of each technique are examined, and the future applications of the recently developed techniques are also explored.
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