Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers.
Molecular epidemiological study of hepatitis B virus in blood donors from five Chinese blood centers.
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DOI：
10.1007/s00705-012-1331-x
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发表时间：
2012-09
影响因子：
2.7
通讯作者：
Shan, Hua
中科院分区：
文献类型：
--
作者：
Liu, Yu;Wang, Jingxing;Huang, Yi;Yang, Tonghan;Guo, Xiaoming;Li, Julin;Wen, Guoxin;Yun, Zhongqiao;Zeng, Peibin;He, Miao;Xu, Min;Liu, Gui;Ke, Ling;Wright, David;Liu, Jing;Nelson, Kenrad;Shan, Hua
尽管B肝炎病毒（HBV）感染者的基因变异性已得到广泛研究，但关于中国献血员HBV毒株的基因型、亚型和S区突变的报道有限。在这项研究中，245份血液样本从中国五个不同地理位置的血液中心收集的HBsAg阳性献血者。扩增HBV S区，确定HBV基因型和亚型。比对S区氨基酸序列，确定与免疫失败和HBsAg检测相关的突变。在245份样本中，228份（93%）成功进行了基因分型。基因型B、C、D和A分别占58.8%、21.9%、6.6%和3.95%。HBV抗原亚型分布为adw（67. 6%）、adr（23. 3%）和ayw（8. 7%）。在228份样本中，39份（17.1%）在S区的主要亲水区（MHR）中存在突变。本研究表明，HBV基因型/亚型B/adw是中国HBV感染者中最常见的流行株，其次是C/adr。MHR突变体在HBV感染的献血者中的发生以及在采集单位中可能无法检测到其中一些突变体对输血安全构成威胁。
Although the genetic variability of hepatitis B virus (HBV) in HBV-infected patients has been extensively studied, reports on genotypes, subtypes and mutations in the S region of HBV strains from Chinese blood donors are limited. In this study, 245 blood samples from HBsAg-positive blood donors were collected from five geographically diverse blood centers in China. The S region of HBV was amplified, and the HBV genotype and subtype were determined. The amino acid sequences of the S region were aligned, and mutations related to the failure of immunization and HBsAg detection were determined. Of the 245 samples, 228 (93 %) were genotyped successfully. We found that genotypes B, C, D and A accounted for 58.8 %, 21.9 %, 6.6 % and 3.95 % of the isolates, respectively. The distribution of HBV antigen subtypes was as follows: adw (67.6 %), adr (23.3 %) and ayw (8.7 %). Mutations were present in 39 (17.1 %) of 228 samples in the major hydrophilic region (MHR) of the S region. This study demonstrated that HBV genotype/subtype B/adw was the most frequent strain circulating in HBV-infected Chinese blood donors, followed by C/adr. The occurrence of MHR mutants in HBV-infected blood donors and the potential failure to detect some of them in collected units poses a threat to transfusion safety.
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影响因子：
4.1
作者：
Locarnini, Stephen A
通讯作者：
Locarnini, Stephen A
影响因子：
6.4
作者：
Ogura, Y;Kurosaki, M;Sato, C
通讯作者：
Sato, C
影响因子：
6.4
作者：
Liang, Xiaofeng;Bi, Shengli;Wang, Yu
通讯作者：
Wang, Yu
影响因子：
6.3
作者：
Kobayashi, M;Arase, Y;Kumada, H
通讯作者：
Kumada, H
影响因子：
12.7
作者：
Echevarria, Jose M.;Avellon, Ana
通讯作者：
Avellon, Ana