The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.
The tissue distribution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro.
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小鼠B7-2 costimulator的组织分布：在原位和体外成熟期间在树突状细胞上表达丰富的表达。
DOI：
10.1084/jem.180.5.1849
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发表时间：
1994-11-01
影响因子：
15.3
通讯作者：
Steinman, R. M.
中科院分区：
文献类型：
--
作者：
Inaba, K.;Witmer-Pack, M.;Inaba, M.;Hathcock, K. S.;Sakuta, H.;Azuma, M.;Yagita, H.;Okumura, K.;Linsley, P. S.;Ikehara, S.;Muramatsu, S.;Hodes, R. J.;Steinman, R. M.
B7 - 2是最近发现的CTLA - 4/CD28、T细胞信号系统的第二种配体。我们使用GL - 1大鼠单克隆抗体（mAb）监测了B7 - 2在小鼠白细胞上的表达，重点关注树突状细胞。通过细胞荧光分析，在从脾脏、胸腺、腹腔、皮肤、骨髓和血液中分离出的大多数细胞类型上很少或没有检测到B7 - 2。然而，在培养过程中B7 - 2的表达可以上调。对于表皮和脾脏树突状细胞，它们在短期培养过程中对T细胞具有高度免疫刺激性，B7 - 2的上调非常显著且不需要额外的刺激。与巨噬细胞和B细胞相反，脂多糖不会上调树突状细胞上的B7 - 2水平。通过间接免疫标记，GL - 1 mAb的染色水平超过了大鼠mAbs对其他几种表面分子的染色水平，这些分子包括细胞间黏附分子1、B7 - 1、CD44和CD45，以及新的仓鼠mAbs对CD40、CD48和B7 - 1/CD80的染色水平。在这些辅助分子中，B7 - 2是在培养过程中增加的主要种类，这意味着B7 - 2在树突状细胞的功能成熟中起关键作用。B7 - 2是小鼠树突状细胞上主要的（>90%）CTLA - 4配体。当我们将GL - 1应用于十几个不同器官的组织切片时，在许多切片中发现了B7 - 2抗原的清晰染色。在肝脏枯否细胞、心脏和肺的间质细胞以及食管黏膜下层的轮廓中观察到B7 - 2染色。在肾脏和肠道的非淋巴区域B7 - 2染色极少，在大脑中完全没有观察到。在舌头中，口腔上皮中只有极少数树突状细胞呈B7 - 2阳性，但反应性细胞散布在肌肉的间质空间中。在所有淋巴组织中，GL - 1强烈染色某些由树突状细胞和巨噬细胞占据的特定区域。对于树突状细胞，这些区域包括胸腺髓质、脾动脉周围鞘和淋巴结深皮质；对于巨噬细胞，富含B7 - 2的区域包括脾边缘区和淋巴结被膜下皮质。静脉注射GL - 1 mAb可使脾脏B7 - 2阳性细胞被标记。在混合白细胞反应中，树突状细胞对T细胞的刺激（DNA合成）被GL - 1显著（35 - 65%）阻断。（摘要截断至400字）
B7-2 is a recently discovered, second ligand for the CTLA-4/CD28, T cell signaling system. Using the GL-1 rat monoclonal antibody (mAb), we monitored expression of B7-2 on mouse leukocytes with an emphasis on dendritic cells. By cytofluorography, little or no B7-2 was detected on most cell types isolated from spleen, thymus, peritoneal cavity, skin, marrow, and blood. However, expression of B7-2 could be upregulated in culture. In the case of epidermal and spleen dendritic cells, which become highly immunostimulatory for T cells during a short period of culture, the upregulation of B7-2 was dramatic and did not require added stimuli. Lipopolysaccharide did not upregulate B7-2 levels on dendritic cells, in contrast to macrophages and B cells. By indirect immunolabeling, the level of staining with GL-1 mAb exceeded that seen with rat mAbs to several other surface molecules including intercellular adhesion molecule 1, B7-1, CD44, and CD45, as well as new hamster mAbs to CD40, CD48, and B7-1/CD80. Of these accessory molecules, B7-2 was a major species that increased in culture, implying a key role for B7-2 in the functional maturation of dendritic cells. B7- 2 was the main (> 90%) CTLA-4 ligand on mouse dendritic cells. When we applied GL-1 to tissue sections of a dozen different organs, clear-cut staining with B7-2 antigen was found in many. B7-2 staining was noted on liver Kupffer cells, interstitial cells of heart and lung, and profiles in the submucosa of the esophagus. B7-2 staining was minimal in the kidney and in the nonlymphoid regions of the gut, and was not observed at all in the brain. In the tongue, only rare dendritic cells in the oral epithelium were B7-2+, but reactive cells were scattered about the interstitial spaces of the muscle. In all lymphoid tissues, Gl-1 strongly stained certain distinct regions that are occupied by dendritic cells and by macrophages. For dendritic cells, these include the thymic medulla, splenic periarterial sheaths, and lymph node deep cortex; for macrophages, the B7-2-rich regions included the splenic marginal zone and lymph node subcapsular cortex. Splenic B7-2+ cells were accessible to labeling with GL-1 mAb given intravenously. Dendritic cell stimulation of T cells (DNA synthesis) during the mixed leukocyte reaction was significantly (35-65%) blocked by GL-1.(ABSTRACT TRUNCATED AT 400 WORDS)
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DOI：
10.1084/jem.153.6.1666
发表时间：
1981-06-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Barclay AN;Mayrhofer G
通讯作者：
Mayrhofer G
DOI：
10.1084/jem.178.6.2185
发表时间：
1993-12-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Freeman GJ;Borriello F;Hodes RJ;Reiser H;Gribben JG;Ng JW;Kim J;Goldberg JM;Hathcock K;Laszlo G
通讯作者：
Laszlo G
DOI：
10.1084/jem.157.4.1101
发表时间：
1983-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Austyn JM;Steinman RM;Weinstein DE;Granelli-Piperno A;Palladino MA
通讯作者：
Palladino MA
影响因子：
15.3
作者：
Harding, F A;Allison, J P
通讯作者：
Allison, J P
B7-1和B7-2共刺激配体的比较分析：表达和功能。
DOI：
10.1084/jem.180.2.631
发表时间：
1994-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hathcock KS;Laszlo G;Pucillo C;Linsley P;Hodes RJ
通讯作者：
Hodes RJ