A high utility integrated map of the pig genome.
A high utility integrated map of the pig genome.
复制标题
猪基因组的高实用性集成图。
DOI：
10.1186/gb-2007-8-7-r139
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发表时间：
2007
期刊：
影响因子：
12.3
通讯作者：
Rogers, Jane
中科院分区：
文献类型：
--
作者：
Humphray, Sean J.;Scott, Carol E.;Clark, Richard;Marron, Brandy;Bender, Clare;Camm, Nick;Davis, Jayne;Jenks, Andrew;Noon, Angela;Patel, Manish;Sehra, Harminder;Yang, Fengtang;Rogatcheva, Margarita B.;Milan, Denis;Chardon, Patrick;Rohrer, Gary;Nonneman, Dan;de Jong, Pieter;Meyers, Stacey N.;Archibald, Alan;Beever, Jonathan E.;Schook, Lawrence B.;Rogers, Jane
一个新的物理图谱的牛基因组已经构建了整合数据的遗传和辐射杂交图谱，一个新的牛BAC图谱，与牛基因组草图组装。家猪越来越多地被用作人类疾病的模型系统。它对肉类蛋白质生产也具有重大的经济重要性。物理克隆图谱支持大规模基因组测序，并使许多基因组的克隆工作成为可能。比较遗传图谱表明，猪和人之间的结构相似性比小鼠和人之间的结构相似性更高，我们利用人和猪之间的这种密切关系来促进图谱构建。在这里，我们报告的构建最高度连续的细菌人工染色体（BAC）的地图的任何哺乳动物基因组，猪（猪）基因组。该图谱为跨基因组的高质量锚定序列的生成和组装提供了模板。物理图谱整合了先前的界标图谱与来自4个BAC文库的超过260，000个BAC的限制性指纹和BAC末端序列，并利用与人类基因组的比对来改善克隆重叠群的连续性和局部排序。我们估计，超过98%的常染色质的18个猪常染色体和X染色体沿着与本地覆盖的Y是代表在172重叠群，与染色体13（218 Mb）由一个单一的重叠群。该图谱可通过pre-Ensembl访问，在那里可以找到标记和序列数据的链接。该图谱将使基因的即时电子定位克隆成为可能，使猪研究界受益，并进一步促进猪作为人类疾病的替代动物模型的使用。克隆图谱和BAC末端序列数据也可以帮助支持其他偶蹄动物的图谱和基因组序列的组装。
A new physical map of the bovine genome has been constructed by integrating data from genetic and radiation hybrid maps, and a new bovine BAC map, with the bovine genome draft assembly. The domestic pig is being increasingly exploited as a system for modeling human disease. It also has substantial economic importance for meat-based protein production. Physical clone maps have underpinned large-scale genomic sequencing and enabled focused cloning efforts for many genomes. Comparative genetic maps indicate that there is more structural similarity between pig and human than, for example, mouse and human, and we have used this close relationship between human and pig as a way of facilitating map construction. Here we report the construction of the most highly continuous bacterial artificial chromosome (BAC) map of any mammalian genome, for the pig (Sus scrofa domestica) genome. The map provides a template for the generation and assembly of high-quality anchored sequence across the genome. The physical map integrates previous landmark maps with restriction fingerprints and BAC end sequences from over 260,000 BACs derived from 4 BAC libraries and takes advantage of alignments to the human genome to improve the continuity and local ordering of the clone contigs. We estimate that over 98% of the euchromatin of the 18 pig autosomes and the X chromosome along with localized coverage on Y is represented in 172 contigs, with chromosome 13 (218 Mb) represented by a single contig. The map is accessible through pre-Ensembl, where links to marker and sequence data can be found. The map will enable immediate electronic positional cloning of genes, benefiting the pig research community and further facilitating use of the pig as an alternative animal model for human disease. The clone map and BAC end sequence data can also help to support the assembly of maps and genome sequences of other artiodactyls.
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影响因子：
2.5
作者：
Mikawa, A;Suzuki, H;Hamasima, N
通讯作者：
Hamasima, N
影响因子：
56.9
作者：
Murphy, WJ;Larkin, DM;Lewin, HA
通讯作者：
Lewin, HA
影响因子：
5.8
作者：
Carver, TJ;Rutherford, KM;Parkhill, J
通讯作者：
Parkhill, J
影响因子：
7
作者：
Krzywinski, M;Wallis, J;Schein, J
通讯作者：
Schein, J
影响因子：
2.2
作者：
Nishitai, R;Ikai, I;Yamaoka, Y
通讯作者：
Yamaoka, Y