Phage display as a promising approach for vaccine development.
Phage display as a promising approach for vaccine development.
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DOI：
10.1186/s12929-016-0285-9
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发表时间：
2016-09-29
影响因子：
11
通讯作者：
Majidi J
中科院分区：
文献类型：
--
作者：
Aghebati-Maleki L;Bakhshinejad B;Baradaran B;Motallebnezhad M;Aghebati-Maleki A;Nickho H;Yousefi M;Majidi J
噬菌体是细菌宿主的特异性拮抗剂。这些病毒实体作为最佳疫苗递送载体已经引起了越来越多的兴趣。由于噬菌体在恶劣环境条件下高度稳定、简单且廉价的大规模生产以及有效的佐剂能力，噬菌体与疫苗设计非常匹配。噬菌体疫苗具有有效的免疫刺激作用，并呈现出高安全性，因为这些病毒在长期进化期间与哺乳动物身体形成了恒定的关系。噬菌体展示技术的诞生是噬菌体疫苗发展的一个转折点。噬菌体展示疫苗通过在免疫原性噬菌体颗粒的表面上表达抗原的多个拷贝来制备，从而引发强大且有效的免疫应答。此外，产生具有高度多样化的随机化配体库的组合肽文库的能力已经将噬菌体展示转化为用于鉴定针对不同疾病（特别是微生物感染）的潜在疫苗候选物的简单、通用和高通量筛选方法。这些文库可以通过称为生物淘选的基于亲和选择的策略针对各种各样的靶标进行方便地筛选，以选择具有高抗原性和免疫原性的模拟表位。此外，它们可以针对恢复期个体的抗血清进行淘选，以识别病原体相关表位的新型肽模拟物。噬菌体展示为寻找疫苗发现和生产的新策略带来了巨大的希望，目前的突破为开发不同的基于噬菌体的疫苗平台带来了光明的未来。
Bacteriophages are specific antagonists to bacterial hosts. These viral entities have attracted growing interest as optimal vaccine delivery vehicles. Phages are well-matched for vaccine design due to being highly stable under harsh environmental conditions, simple and inexpensive large scale production, and potent adjuvant capacities. Phage vaccines have efficient immunostimulatory effects and present a high safety profile because these viruses have made a constant relationship with the mammalian body during a long-standing evolutionary period. The birth of phage display technology has been a turning point in the development of phage-based vaccines. Phage display vaccines are made by expressing multiple copies of an antigen on the surface of immunogenic phage particles, thereby eliciting a powerful and effective immune response. Also, the ability to produce combinatorial peptide libraries with a highly diverse pool of randomized ligands has transformed phage display into a straightforward, versatile and high throughput screening methodology for the identification of potential vaccine candidates against different diseases in particular microbial infections. These libraries can be conveniently screened through an affinity selection-based strategy called biopanning against a wide variety of targets for the selection of mimotopes with high antigenicity and immunogenicity. Also, they can be panned against the antiserum of convalescent individuals to recognize novel peptidomimetics of pathogen-related epitopes. Phage display has represented enormous promise for finding new strategies of vaccine discovery and production and current breakthroughs promise a brilliant future for the development of different phage-based vaccine platforms.
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DOI：
10.3390/molecules16053499
发表时间：
2011-04-26
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Castel G;Chtéoui M;Heyd B;Tordo N
通讯作者：
Tordo N
DOI：
10.1073/pnas.241655998
发表时间：
2002-02-05
影响因子：
11.1
作者：
Arap, W;Haedicke, W;Ruoslahti, E
通讯作者：
Ruoslahti, E
影响因子：
--
作者：
Clark, JR;March, JB
通讯作者：
March, JB
影响因子：
3.5
作者：
Becker, Martin;Felsberger, Andre;Hust, Michael
通讯作者：
Hust, Michael
影响因子：
82.9
作者：
Baral, Toya Nath;Magez, Stefan;De Baetselier, Patrick
通讯作者：
De Baetselier, Patrick